ISOLATE II Genomic DNA Kit

ISOLATE II Genomic DNA Kit provides a simple, efficient column-based method for the isolation of genomic DNA from a wide variety of materials, without the need for hazardous reagents such as phenol

Category:DNA Extraction Kits Brand: Bioline

Description

Cat. No.	Size
BIO-52065	10 Preps
BIO-52066	50 Preps
BIO-52067	250 Preps

Product Highlights

Fast – streamlined protocol for consistent recovery of genomic DNA in as little as 20 minutes
High-performance – extraction of high-purity DNA, ideal for use in all downstream applications
High-yield – efficient sample lysis and column-binding for high-yield recovery of genomic DNA from limiting amounts of sample
Flexible – reliable extraction of genomic DNA from a very broad range of human, animal and microbial samples

By combining Proteinase K lysis with the speed and convenience of silica membrane purification, ISOLATE II Genomic DNA Kit provides a fast method for the purification of high-quality genomic DNA from a variety of starting materials.

By combining Proteinase K lysis with the speed and ease-of-use of silica-membrane purification, the ISOLATE II Genomic DNA Kit provides a fast method for the purification of high-quality genomic DNA from a variety of starting materials, such as mouse or rat tails, bacteria, yeast, dried blood spots, genomic/viral DNA from blood, hair follicles, paraffin-embedded issue (FFPE), insects, dental swabs, buccal swabs, stool viruses (e.g. CMV), Mycobacterium tuberculosis or Legionella pneumophila in sputum or bronchoalveolar lavage, EHEC bacteria in food, bacterial DNA (e.g. Chlamydia trachomatis) from cultures, biological fluids or clinical specimens, bacterial DNA (e.g. Borrelia burgordferi) and viral DNA (e.g. CMV) from urine.

ISOLATE II Genomic DNA Kit has been designed to deliver optimal performance in qPCR in tandem with SensiFAST Real-Time PCR Kits and in end-point PCR with MyTaq DNA Polymerase.

Applications

qPCR
End-point PCR
Southern, dot and slot blotting
Genotyping
Restriction digestion
Next Generation Sequencing
Bisulfite conversion/methylation analysis

Specifications

Components

Reagent	10 Preps	50 Preps	250 Preps
ISOLATE II Genomic DNA Spin Columns	10	50	250
Collection Tubes (2 mL)	20	100	500
Lysis Buffer GL	5 mL	20 mL	100 mL
Buffer G3	10 mL	15 mL	75 mL
Wash Buffer GW1	6 mL	30 mL	150 mL
Wash Buffer GW2 (concentrate)	6 mL	12 mL	50 mL
Elution Buffer G	13 mL	13 mL	60 mL
Proteinase K (lyophilized)	6 mg	30 mg	2 x 75 mg
Proteinase K Buffer PR	1.8 mL	1.8 mL	8 mL
Bench Protocol Sheet	1	1	1

Storage & Stability

All components should be stored dry and at room temperature.

When stored under the recommended conditions and handled correctly, full activity of reagents is retained until the expiry date indicated on the outer box label.