ISOLATE II Plant DNA Kit

ISOLATE II Plant DNA Kit provides a simple, efficient column-based method for the isolation of genomic DNA from a wide variety of plant materials, without the need for hazardous reagents such as phenol

Category:DNA Extraction Kits Brand: Bioline

Description

Cat. No.	Size
BIO-52068	10 preps
BIO-52069	50 preps
BIO-52070	250 preps

Product Highlights

Fast – streamlined protocol for reliable recovery of genomic DNA in as little as 30 minutes
High-performance – extraction of high-quality DNA, ideal for use in all downstream applications
Efficient – optimized lysis conditions and column matrix for improved recovery of genomic DNA from a wide range of plant samples
Versatile – a choice of two lysis buffers ensures excellent genomic DNA recovery from any plant material
Convenient – kit includes all necessary components, including filters (shredders) and RNase A
Safe – no hazardous phenol/chloroform extraction

By combining the stringency of CTAB lysis with the speed and ease-of-use of silica-membrane purification, ISOLATE II Plant DNA Kit provides a fast method for the purification of high-quality genomic DNA from most plant cells, particularly those rich in polysaccharides, including leaves, bark, roots and fruits as well as dung, animal-fecal, soil and compost samples.

Some plant tissues do not lyse well in CTAB and so an SDS-based lysis buffer is also provided as an alternative. Residual amounts of RNA remaining can be removed using RNase treatment during lysis with the RNase A supplied in the kit.

ISOLATE II Plant DNA Kit shows excellent recovery of plant DNA when different homogenization techniques are used. The silica membrane in the columns is optimized to improve DNA binding to give high-yields of high-quality DNA, even from difficult samples such as freeze-dried budding leaves. The extracted DNA is ready to use for subsequent downstream reactions such as PCR.

ISOLATE II Plant DNA Kit has been designed to deliver optimal performance in qPCR in tandem with SensiFAST Real-Time PCR Kits and in end-point PCR with MyTaq DNA Polymerase.

Applications

qPCR
End-point PCR
Southern, dot and slot blotting
Genotyping
Restriction digestion
Next generation sequencing
Bisulfite conversion/methylation analysis

Specifications

Components

Reagent	10 Preps	50 Preps	250 Preps
ISOLATE II Filters	10	50	250
ISOLATE II Plant Columns	10	50	250
Collection Tubes (2 mL)	20	100	500
Lysis Buffer PA1	5 mL	25 mL	125 mL
Lysis Buffer PA2	4 mL	20 mL	100 mL
Precipitation Buffer PL3	1 mL	10 mL	25 mL
Binding Buffer PB	6 mL	30 mL	125 mL
Wash Buffer PAW1	6 mL	30 mL	125 mL
Wash Buffer PAW2	6 mL	25 mL	50 mL
Elution Buffer PG	13 mL	13 mL	30 mL
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