ISOLATE II PCR and Gel Kit

Simple, efficient membrane-based method for the purification of DNA from PCR reactions and from TAE & TBE agarose gels, without the need for hazardous reagents or ethanol precipitation

Category:DNA Extraction Kits Brand: Bioline

Description

Cat. No.	Size
BIO-52058	10 Preps
BIO-52059	50 preps
BIO-52060	250 preps

Product Highlights

Fast – streamlined protocol for simple recovery of PCR products in 10 minutes and DNA from gel slices in 20 minutes
Efficient – column matrix developed for recovery rates of between 70% and 95%
High-performance – extraction of consistently high-quality DNA fragments, ideal for use in all downstream applications
Versatile – single buffer promotes effective DNA recovery from both TAE and TBE agarose gels
Safe – no hazardous phenol/chloroform extraction

ISOLATE II PCR and Gel Kit is the simplest option for the purification of PCR products and for the isolation of DNA fragments from TAE and TBE agarose gel slices. A fast and easy-to-follow protocol is given for each application.

PCR products can be purified in 10 minutes using simple binding and elution steps. Concentrated PCR products ranging between 60 bp and 15 kb can be eluted, removing primers, nucleotides, enzymes, mineral oil, salts and other impurities.

DNA fragments between 50 bp and 20 kb can be extracted from agarose gel slices in 20 minutes using a color indicator to help maintain optimal pH and identify undissolved agarose.

ISOLATE II PCR and Gel Kit has been designed to deliver optimal performance in downstream applications, including transformations, cloning, sequencing and restriction analysis.

Applications

Cloning
Ligation
Restriction digestion
Fluorescence sequencing
Labeling
PCR
Transfection
In vitro transcription

Specifications

Components

Reagent	10 Preps	50 Preps	250 Preps
ISOLATE II PCR and Gel Columns (yellow)	10	50	250
Collection Tubes	10	50	250
Binding Buffer CB	10 mL	40 mL	200 mL
Wash Buffer CW (Concentrate)	6 mL	25 mL	2 x 50 mL
Elution Buffer C	13 mL	13 mL	30 mL
Bench Protocol Sheet	1	1	1

Storage & Stability

All components should be stored dry and at room temperature.

When stored under the recommended conditions and handled correctly, full activity of reagents is retained until the expiry date indicated on the outer box label.