ISOLATE II Plasmid Mini Kit

Highly efficient purification of plasmid DNA by combining fast SDS/alkaline lysis of the starting material with the speed and purity of silica-membrane column purification.

Category:DNA Extraction Kits Brand: Bioline

Description

Cat. No.	Size
BIO-52055	10 preps
BIO-52056	50 preps
BIO-52057	250 Preps

Product Highlights

Fast – streamlined protocol for extraction of eighteen plasmid samples in as little as 15 minutes
Efficient – rapid SDS/alkaline lysis and efficient column-binding for high-yield recovery of all plasmid molecules
High-performance – efficient extraction of high-purity plasmid DNA, ideal for use in all downstream applications
Flexible – reliable recovery of high- and low-copy plasmids from all gram-positive bacteria

The ISOLATE II Plasmid Mini Kit provides a simple, efficient column-based method for the isolation of plasmid DNA from bacterial cultures, without the need for hazardous reagents such as phenol.

By combining SDS/alkaline lysis with the speed and ease-of-use of silica-membrane purification, the ISOLATE II Plasmid Mini Kit provides a fast method for the purification of high-quality plasmid DNA. Separate protocols are provided for the isolation of high-copy plasmids, the isolation of low-copy plasmid, P1 constructs and cosmid DNA from E. coli and the isolation of plasmids from gram-positive bacteria such as Bacillus, Staphylococcus, Bifidobacteria or Corynebacteria.

The ISOLATE II Plasmid Mini Kit has been designed to deliver optimal performance in cloning, sequencing as well as end-point PCR alongside any enzyme from the Meridian PCR portfolio, including MyTaq DNA Polymerase. Additionally, the ISOLATE II Plasmid Mini Kit can be used in tandem with the SensiFAST Real-Time PCR Kits for high-performance qPCR.

Applications

Cloning
Sequencing
Restriction digestion
Labeling
End-point PCR
qPCR
Transfection
Fluorescent sequencing
In vitro transcription

Specifications

Components

Reagent	10 Preps	50 Preps	250 Preps
ISOLATE II Plasmid Mini Spin Columns	10	50	250
Collection Tubes (2 mL)	10	50	250
Resuspension Buffer P1	5 mL	15 mL	75 mL
Lysis Buffer P2	5 mL	15 mL	100 mL
Neutralization Buffer P3	5 mL	20 mL	100 mL
Wash Buffer PW1	6 mL	30 mL	2 x 75 mL
Wash Buffer PW2	6 mL	12 mL	2 x 25 mL
Elution Buffer P	13 mL	13 mL	60 mL
RNase A (lyophilized)	2.5 mg	6 mg	30 mg
Bench Protocol Sheet	1	1	1

Storage & Stability

All kit components should be stored dry and at room temperature. When stored under the recommended conditions and handled correctly, full activity of kit reagents is retained until the expiry date indicated on the outer box label.

Note: RNase A is supplied lyophilized. On addition of Resuspension Buffer P1, the RNase A solution should be kept stored at 4°C. When stored at 4°C. the RNase A solution is stable for at least 6 months.