ISOLATE II Fecal DNA Kit

Highly efficient purification of host-cell and microbial DNA by combining fast lysis of the starting material in the presence of guanidinium thiocyanate with the speed and purity of silica-membrane column purification.

Category:DNA Extraction Kits Brand: Bioline

Description

Cat. No.	Size
BIO-52082	50 Preps

Product Highlights

Fast – streamlined protocol for extraction of host cell and microbial DNA in as little as 15 minutes
High-performance – recovery of consistently high-quality DNA, ideal for use in all downstream applications
Efficient – rapid and effective lysis of fecal samples by bead beating for excellent recovery of DNA
Flexible – reliable recovery of host and microbial DNA from human and a broad range of animal species

The ISOLATE II Fecal DNA Kit provides a simple, efficient column-based method for the isolation of DNA from a wide variety of fecal samples, without the need for hazardous reagents such as phenol.

By combining the stringency of guanidinium-thiocyanate lysis and high-density bashing beads and the speed and ease-of-use of silica-membrane purification to remove humic acids/polyphenols, the ISOLATE II Fecal DNA Kit provides a fast method for the purification of high-quality bacterial/protist DNA from a variety of fecal samples including humans, birds, rats, mice, rabbits, cattle, etc. as well as host DNA.

The ISOLATE II Fecal DNA Kit has been designed to purify samples ideal for use in end-point PCR alongside any enzyme from the Meridian PCR portfolio, including MyTaq DNA Polymerase and in qPCR using the the SensiFAST Real-Time PCR Kits.

Applications

End-point PCR
qPCR
Southern, dot and slot blotting
Genotyping
Next Generation Sequencing
Bisulfite conversion/methylation analysis

Specifications

Components

Reagent	50 Preps	100 Preps
Lysis Buffer	1 x 40 mL	2 x 40 mL
DNA Pre-Wash Buffer	1 x 15 mL	2 x 15 mL
Fecal DNA Wash Buffer	1 x 50 mL	2 x 50 mL
Fecal DNA Binding Buffer	1 x 100 mL	2 x 100 mL
DNA Elution Buffer	1 x 10 mL	2 x 10 mL
Fecal Prep Solution	1 x 30 mL	2 x 30 mL
Bashing Beads Lysis Tubes	1 x 50	2 x 50
Spin II A Filters	1 x 50	2 x 50
Spin II B Filters	1 x 50	2 x 50
Spin Columns	1 x 50	2 x 50
Collection Tubes	200	2 x 200
Bench Protocol Sheet	1	1

Storage & Stability

All components should be stored dry and at room temperature. When stored under the recommended conditions and handled correctly, full activity of reagents is retained until the expiry date indicated on the outer box label.