Description
Isopropyl-ß-D-thiogalactoside, IPTG is a chemical analogue of galactose that cannot be cleaved by ß-galactosidase. Functioning as an analogue, IPTG binds and inhibits the powerful lac repressor, strongly inducing the production of ß-galactosidase..IPTG is used in conjunction with X-Gal for detection of lac+ colonies or cells in a colorimetric assay, in order to distinguish recombinants (white) from non recombinants (blue) in cloning strategies using vectors like Lamb-da-11, M13m-p18 and 19, pUC18 and 19, pUR222 containing the lacZ gene. For more detailed information see X-Gal.
