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HomeShopLaboratory consumablesPCRPCR Plates/TubesFrameStar® 96 Well Skirted Optical Bottom PCR Plate
FrameStar® 96 Well Skirted Optical Bottom PCR Plate
4ti-0970 | FrameStar 96 Well Skirted Optical Bottom PCR Plate | Front

FrameStar® 96 Well Skirted Optical Bottom PCR Plate

96 well skirted plates unique on the market, suitable for use in both microscopy and PCR. Low profile wells, especially recommended for reaction volumes <20µl. Rigid frame eliminates warping and distortion during PCR. Ideal for use with robotic systems.

  • PP wells, PC frame; cut corner H1
  • <100µl working volume, 180µl total well capacity
  • Available barcoded on request
  • Ideal for single cell sorting followed by (q)PCR
SKU: 4ti-0970 Category:PCR Plates/Tubes Brand: Azenta
  • Description
  • Specifications
  • Downloads

Description

Product Details

We have now extended our FrameStar range to include PCR plates with optically clear flat bottom wells for microscopy applications. The FrameStar 96 Well Skirted Optical Bottom PCR Plates (next to their counterpart within our Individual Access range, 4ti-0970/RA) are currently unique in the market, being suitable for use in both microscopy and PCR.

Optical bottom plates are ideal for applications requiring single cell sorting followed by molecular biology techniques such as (q)PCR and sequencing. The flat bottoms enable excellent stackability, making these plates well suited for small sample volume storage such as compound libraries, with no risk of damaging the seal of the plate below. Additionally, the small well volume enables excellent sample recovery.

Key Features

Quality

  • Free from DNase, RNase, human genomic DNA, and endotoxin/pyrogen
  • Stackable

Wells

  • Ultra-smooth, uniform, thin-walled polypropylene wells for optimum PCR and real-time (RT-qPCR) results
  • <0.1ml (100µl) working volume, 0.18ml (180µl) total well capacity
  • Low profile wells, shorter than “standard” profile wells, to decrease the “dead space” between the heated lid of the thermal cycler and the sample in the well
  • Eliminates condensation forming on the side wall of the tubes, preventing reduction in PCR volume and increasing the efficiency of the reaction

Frame

  • Rigid polycarbonate frame for added mechanical stability
  • Significantly reduced thermal expansion and sample evaporation for improved consistency in PCR results
  • Improved seal integrity due to thermal stability of frame
  • Alphanumeric grid reference to aid well and sample identification

Use

  • Suitable for microscopy and small volume sample storage
  • Recommended for low volume PCR
  • Compatible with standard multichannel pipettes
  • Ideal for use with robotic systems

Options

  • Also available as an Individual Access plate (4ti-0970/RA) with individually detachable and sealable wells
  • Available barcoded upon request; learn more about our barcoding options

Ordering Information

Part Numbers

 

FrameStar 96 Well Skirted Optical Bottom PCR Plate
Low profile, flat optical bottom, 0.1ml clear polypropylene wells, clear polycarbonate frame, cut corner H1, working volume: <100µl, total well capacity: 180µl
4ti-0970 clear wells, clear frame; 50 plates

Compatibility

AGILENT/STRATAGENE

  • Thermal Cyclers (96 well block): Robocycler Gradient
  • qPCR Cyclers (96 well block): AriaMx

ANALYTIK JENA/BIOMETRA

  • Thermal Cyclers (96 well block): Flexcycler2 | T1 Thermocycler | Tgradient | Tone | Tadvanced | TProfessional (Standard/Basic) Gradient / XL | Trobot 96 | SpeedCycler2 (SP, SPR)

BIO-RAD

  • Thermal Cyclers (96 well block): C1000 Touch | S1000
  • qPCR Cyclers (96 well block): CFX96 Touch | CFX 96 Touch Deep Well | CFX connect

BIO-RAD MJ RESEARCH

  • Thermal Cyclers (96 well block): PCT100™/200™/220™/221™/225™/240™
  • qPCR Cyclers (96 well block): Chromo4™ | Opticon2™

EPPENDORF

    • Thermal Cyclers (96 well block): MasterCycler® ep/ep gradient/Pro/Pro S/nexus/nexus gradient/nexus SX1/nexus GSX1

  • qPCR Cyclers (96 well block): MasterCycler™ ep realplex

GE HEALTHCARE/AMERSHAM

    • Sequencers (96 well block): MegaBACE™ 500 | MegaBACE™ 1000 mark 2

PEQLAB/VWR

    • Thermal Cyclers (96 well block): peqSTAR 96X

SENSOQUEST

    • Thermal Cyclers (96 well block): Labcycler

TECHNE
    • Thermal Cyclers (96 well block): TC412 | TC512 | Genius | Genius Quad | Touchgene | Touchgene Gradient | Flexigene

  • qPCR Cyclers (96 well block): Quantica

Specifications

FrameStar 96 Well Skirted Optical Bottom PCR Plate
Plate length 127.70 ± 0.25mm
Plate width 85.48 ± 0.25mm
Plate height 16.10 ± 0.25mm
Well depth 12 ± 0.10mm
Well diameter 5.46 ± 0.10mm
Total well capacity 180µl
Working volume <0.1ml (100µl)
Distance to center of A1 from top edge 11.24 ± 0.25mm
Distance to center of A1 from left edge 14.38 ± 0.25mm
Pitch (distance between A1 and A2) 9.00mm
Differences in tube format between the standard conical PCR tube and the flat bottom tubes

Figure 1 illustrates the differences in tube format between the standard conical PCR tube and the flat bottom tubes

The surface area of the optical base is 7.74mm2, with a thickness of 0.5mm. The total volume of a conical well is 0.2ml compared to 0.18ml for flat bottom tubes

The primary target application for optical bottom plates is assays that require the use of microscopy, for example, work with single cells including fluorescent imaging and cell sorting

No downloads provided.

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