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HomeShopLaboratory consumablesPCRPCR Plates/TubesFrameStar® 96 Well Semi-Skirted PCR Plate, Roche Style, High Sensitivity
FrameStar® 96 Well Semi-Skirted PCR Plate, Roche Style, High Sensitivity
4ti-0954 | FrameStar 96 Well Semi-Skirted PCR Plate, Roche Style, High Sensitivity | Front
FrameStar® 96 Well Semi-Skirted PCR Plate, Roche Style, High Sensitivity - Image 1

FrameStar® 96 Well Semi-Skirted PCR Plate, Roche Style, High Sensitivity

96 well semi-skirted plates designed for use on Roche LightCycler® 96 & 480 (with 96 well block). Extra white wells improve sensitivity in fluorescent assays. Low profile wells, recommended for reaction volumes <20µl. Rigid frame eliminates warping and distortion during PCR.

  • PP wells, PC frame; cut corner H12
  • <100µl working volume, 200µl total well capacity
  • Available barcoded on request
SKU: 4ti-0954 Category:PCR Plates/Tubes Brand: Azenta
  • Description
  • Specifications
  • Downloads

Description

Product Details

Key Features

Quality

  • Free from DNase, RNase, human genomic DNA, and endotoxin/pyrogen

Wells

  • Ultra-smooth, uniform, thin-walled polypropylene tubes for optimum PCR and real-time (RT-qPCR) results
  • <0.1ml (100µl) working volume, 0.2ml (200µl) total well capacity
  • Low profile wells, shorter than “standard” profile wells, to decrease the “dead space” between the heated lid of the thermal cycler and the sample in the well
  • Eliminates condensation forming on the side wall of the tubes, preventing reduction in PCR volume and increasing the efficiency of the reaction

Frame

  • Rigid polycarbonate frame for added mechanical stability
  • Significantly reduced thermal expansion and sample evaporation for improved consistency in PCR results
  • Improved seal integrity due to thermal stability of frame
  • Alphanumeric grid reference to aid well and sample identification

Use

  • Compatible with the Roche LightCycler® 96 and 480 (with 96 well block)
  • Recommended for low volume PCR
  • Compatible with standard multichannel pipettes
  • Ideal for use with robotic systems

Options

  • Plates are available with a clear polycarbonate frame and extra white wells for improved sensitivity when using fluorescent based assays
  • Combi packs available with qPCR Seal (4ti-0560)
  • Available barcoded upon request; learn more about our barcoding options

Ordering Information

Part Numbers

 

FrameStar 96 Well Semi-Skirted PCR Plate, Roche Style, High Sensitivity Plate
Low profile, 0.1ml polypropylene wells, polycarbonate frame, cut corner H12, working volume: <100µl, total well capacity: 200µl; designed for use on Roche LightCycler® 96 and 480 (with 96 well block); extra white wells for improved sensitivity in fluorescent assays
4ti-0954 extra white wells, clear frame; 50 plates
Combi Packs
4ti-0954/0560 4ti-0954 + qPCR Seal (4ti-0560); 50 plates + 50 seals

Compatibility

ROCHE

  • qPCR Cyclers (96 well block): LC96 | LC480

 

 

Specifications

FrameStar 96 Well Semi-Skirted PCR Plate, Roche Style, High Sensitivity
Plate length 127.70 ± 0.25mm
Plate width 85.48 ± 0.25mm
Plate height 15.60 ± 0.25mm
Well depth 15.10 ± 0.10mm
Well diameter 5.50 ± 0.10mm
Total well capacity 0.2ml (200µl)
Working volume <0.1ml (100µl)
Distance to center of A1 from top edge 11.24 ± 0.25mm
Distance to center of A1 from left edge 14.38 ± 0.25mm
Pitch (distance between A1 and A2) 9.00mm

Ct values comparison

  • Ct values of data run on a Roche LightCycler® 480, comparing 4titude FrameStar Roche Style, High Sensitivity plates (4ti-0954) and Competitor R equivalent plates
  • The new 4titude 4ti-0954 plate out performs the competitor plate with significantly earlier Ct value
FrameStar Roche Style, High Sensitivity Plate Competitor R equivalent plates
Ct value 17.23 20.28
Δ Ct -3.05 0

No downloads provided.

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