Description
The medium defined by Nitsch was used in the production of haploid plants of various species of Nicotiana raised from pollen grains. In this procedure, pollen that were still uninucleate were isolated and then cultured in vitro. Some pollen grains proliferate into embryo-like structures that develop in stages similar to those of zygotic embryos. The plantlets matured and flowered profusely, but did not set seed.
Composition
| Micro Elements | mg/l | µM |
|---|---|---|
| CuSO4.5H2O | 0.025 | |
| FeNaEDTA | 36.70 | |
| H3BO3 | 10.00 | |
| MnSO4.H2O | 18.94 | |
| Na2MoO4.2H2O | 0.25 | |
| ZnSO4.7H2O | 10.00 |
| Macro Elements | mg/l | mM |
|---|---|---|
| CaCl2 | 166.00 | |
| KH2PO4 | 68.00 | |
| KNO3 | 950.00 | |
| MgSO4 | 90.27 | |
| NH4NO3 | 720.00 |
Nitsch J.P. and Nitsch C., Haploid plants from pollen grains, Science 169, 85 (1969). Nitsch J.P., Experimental androgenesis in Nicotiana, Phytomorphology 19, 389 (1969).
Hazard statements
- H315 Causes skin irritation
- H319 Causes serious eye irritation
- H335 May cause respiratory irritation
- H412 Harmful to aquatic life with long lasting effects
Precautionary statements
- P261 Avoid breathing dust/fume/gas/mist/vapours/spray
- P273 Avoid release to the environment
- P302 IF ON SKIN:
- P305 IF IN EYES:
- P351 Rinse continuously with water for several minutes
- P352 Wash with soap and water
