Description
Eriksson medium was developed for cell suspension cultures of Haplopappus gracilis.
An increase in the growth of cell suspensions, especially with small inocula, was achieved by a 10% reduction of the concentration MS microelements, except for Fe and Zn. Equimolar replacement of ZnSO4.-7H2O by ZnNa2EDTA improved cell growth as well. MnEDTA and CoEDTA did not improve the growth of the cell culture. A reduction of Murashige and Skoog NH4NO3 concentration of 1650 to 1200 mg/l and an increase in phosphate to 2.5 mM also stimulated cell growth.
Composition
| Micro Elements | mg/l | µM |
|---|---|---|
| CoCl2.6H2O | 0.0025 | 0.01 |
| CuSO4.5H2O | 0.0025 | 0.01 |
| FeNaEDTA | 36.70 | 100.00 |
| H3BO3 | 0.63 | 10.19 |
| MnSO4.H2O | 1.69 | 10.00 |
| Na2MoO4.2H2O | 0.025 | 0.10 |
| ZnSO4.7H2O | 9.15 | 31.80 |
| Macro Elements | mg/l | mM |
|---|---|---|
| CaCl2 | 332.02 | 2.99 |
| KH2PO4 | 340.00 | 2.50 |
| KNO3 | 1900.00 | 18.79 |
| MgSO4 | 180.54 | 1.50 |
| NH4NO3 | 1200.00 | 14.99 |
| Vitamins | mg/l | µM |
|---|---|---|
| Glycine | 2.00 | 26.64 |
| Nicotinic acid | 0.50 | 4.06 |
| Pyridoxine HCl | 0.50 | 2.43 |
| Thiamine HCl | 0.50 | 1.48 |
Total concentration Micro and Macro elements including -vitamins: 4004.42 mg/l
Eriksson T., Physiol. Plant, 18, 976 (1965).
Hazard statements
- H315 Causes skin irritation
- H319 Causes serious eye irritation
- H335 May cause respiratory irritation
Precautionary statements
- P261 Avoid breathing dust/fume/gas/mist/vapours/spray
- P302 IF ON SKIN:
- P305 IF IN EYES:
- P351 Rinse continuously with water for several minutes
- P352 Wash with soap and water
