MTT

MTT is a water soluble salt of tetrazolium salt yielding a yellowish -solution when prepared in media or salt solutions lacking phenol red. By cleavage of the tetrazolium ring by dehydrogenase enzymes, dissolved MTT is converted into insoluble purple formazan. This water insoluble formazan can be solubilized using isopropanol or other solvents and the dissolved material is measured spectrophotometrically yielding maximum absorbance at 565 nm as a function of concentration of converted dye.