MicroElute® Cycle-Pure & Gel Extraction Kit

MicroElute® Cycle-Pure & Gel Extraction Kit

  • Elution volumes – 10-15 µL
  • Generation of highly concentrated DNA
  • 10 µg DNA binding capacity
  • Rapid isolation – less than 15 minutes

D6294-00S MicroElute® Cycle-Pure & Gel Extraction Kit (Sample Request)

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Description

The E.Z.N.A.® family of products is an innovative system that radically simplifies the extraction and purification of nucleic acids from a variety of sources. The key to this system is the HiBind® matrix that specifically, but reversibly, binds DNA or RNA under
optimized conditions allowing proteins and other contaminants to be removed. Nucleic acids are easily eluted with deionized water or a low salt buffer. The MicroElute® Clean Up system, designed for rapid DNA clean up, includes:

• MicroElute® Cycle-Pure – for direct purification of double or single stranded PCR products (100 bp – 10 kb) from amplification reactions
• MicroElute® Gel Extraction – for extraction of DNA fragments from standard, or low-melt agarose gels in TAE (Tris-acetate/EDTA) or TBE (Tris-borate/EDTA) buffer

Specifications

For Research Use Only. Not for use in diagnostic procedures.

FEATURES SPECIFICATIONS
Downstream application Cloning, In Vitro Transcription, Nucleic Acid Labeling, PCR, Real-Time Quantitative PCR (qPCR), Sequencing, Southern Blotting
Elution volume 10-15 µL
Starting material Agrose gel slice
Starting amount Up to 10 µg DNA
DNA recovered >90% recovery, 70 bp to 20 kb
Processing mode Manual, centrifugation/vacuum
Throughput 1
DNA binding technology Silica MicroElute spin column
Binding capacity 10 μg
Processing time <15 minutes
Special note Low elution volume

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