ISOLATE II RNA Plant Kit

Highly efficient purification of total RNA by combining the stringency of either guanidinium thiocyanate or guanidinium hydrochloride with the speed and purity of silica-membrane column purification.

Category:RNA Extraction Kits Brand: Bioline

Description

Cat. No.	Size
BIO-52076	10 Preps
BIO-52077	50 preps

Product Highlights

RNA was isolated from 20 mg freeze-dried budding leaves of A. thaliana using ISOLATE II RNA Plant Kit. The extracted RNA was diluted in a 2-fold serial dilution (lanes 1-7) and PCR was performed using MyTaq One-Step RT-PCR Kit. The results illustrate the quality of the RNA obtained, as it can be used for very sensitive cDNA synthesis and PCR without further purification.

The ISOLATE II RNA Plant Kit provides a simple, efficient column-based method for the isolation of total RNA from a wide variety of plant materials, including leaves, bark, roots and fruits, without the need for hazardous reagents such as phenol.

By combining the stringency of guanidinium thiocyanate lysis with the speed and ease-of-use of silica-membrane purification, the ISOLATE II RNA Plant Kit provides a fast method for the purification of high-quality total RNA from most plant cells, including plant tissues, leaves, bark, roots and fruits.

Some plant tissues such as maize endosperm and the mycelia of filamentous fungi do not lyse well in guanidinium thiocyanate and can solidify, resulting in poor yields and so guanidinium hydrochloride lysis buffer is also provided as an alternative. Residual amounts of contaminating DNA are selectively removed using a column, however for ultrasensitive applications, remaining DNA can be removed using RNase-free DNase I that is supplied with the kit.

The ISOLATE II RNA Plant Kit has been designed to deliver optimal performance in RT-qPCR in conjunction with either the SensiFAST cDNA Synthesis Kit and SensiFAST Real-Time PCR Kits, or the SensiFAST One-Step Real-Time RT-PCR Kits. Additionally, the ISOLATE II RNA Plant Kit can be used to purify samples prior to RT-PCR amplification using the Tetro cDNA Synthesis Kit and any enzyme from the Meridian PCR portfolio, including MyTaq DNA Polymerase.

Applications

RT-qPCR
End-point RT-PCR
Northern, dot and slot blotting
Array analysis
Poly A+ RNA selection
RNA-Seq

Specifications

Components

Reagent	10 Preps	50 Preps
ISOLATE II Filters	10	50
ISOLATE II RNA Mini Columns	10	50
Collection Tubes (2 mL)	30	150
Collection Tubes (1.5 mL)	10	50
Lysis Buffer RLY	10 mL	25 mL
Lysis Buffer RLS	10 mL	25 mL
Wash Buffer RW1	15 mL	15 mL
Wash Buffer RW2	6 mL	12 mL
Membrane Desalting Buffer MEM	10 mL	25 mL
Reaction Buffer for DNase I RDN	7 mL	7 mL
DNase I, RNase-free (lyophilized)	1 vial	1 vial
RNase-free water	13 mL	13 mL
Bench Protocol Sheet	1	1

Storage & Stability

All components should be stored dry and at room temperature.

When stored under the recommended conditions and handled correctly, full activity of reagents is retained until the expiry date indicated on the outer box label.