ISOLATE II RNA Micro Kit

Highly efficient purification of total RNA from very small samples using an innovative silica-membrane column designed to elute in very small volumes, resulting in high RNA concentrations.

Category:RNA Extraction Kits Brand: Bioline

Description

Cat. No.	Size
BIO-52074	10 Preps
BIO-52075	50 Preps

Product Highlights

Fast – simple extraction of high purity total RNA from twelve samples, ideal for use in all applications, in as little as 40 minutes
High-performance – excellent recovery of high quality RNA from single cells, laser-captured or flow-sorted cells, cryosections and small tissue samples, including biopsies
Safe – no hazardous phenol/chloroform extraction, CsCl centrifugation, LiCl or alcohol precipitation
Concentrated – small elution volumes ensure reaction volumes can be kept low in downstream applications, giving increased reaction efficiency
Convenient – includes all necessary components, including filters (shredders) and DNase I

The ISOLATE II RNA Micro Kit provides a simple, efficient column-based method for the isolation of total RNA from a wide variety of starting materials, without the need for hazardous reagents such as phenol.

By combining the stringency of guanidinium-thiocyanate lysis with the speed and ease-of-use of silica-membrane purification, the ISOLATE II RNA Micro Kit provides a fast method for the purification of high-quality total RNA from single cells, pellets of cultured cells, cryosections, laser-captured or flow sorted cells and small amounts of tissues, for example biopsy materials.

For applications that are sensitive to very small amounts of DNA, residual amounts of DNA remaining can be removed using a convenient on-column DNase treatment with the RNase-free DNase I that is supplied with the kit.

The ISOLATE II RNA Micro Kit has been designed to deliver optimal performance in RT-qPCR in conjunction with either the SensiFAST cDNA Synthesis Kit and SensiFAST Real-Time PCR Kits, or the SensiFAST One-Step Real-Time RT-PCR Kits. Additionally, the ISOLATE II RNA Micro Kit can be used to purify samples prior to PCR and RT-PCR amplification using the Tetro cDNA Synthesis Kit and any enzyme from the Meridian PCR portfolio, including MyTaq DNA Polymerase.

Applications

RT-qPCR
End-point RT-PCR
Northern, dot and slot blotting
Array analysis
RNA protection assays
RNA-Seq

Specifications

Components

Reagent	10 Preps	50 Preps
ISOLATE II Filters	10	50
ISOLATE II RNA Micro Columns & Collection Tubes	10	50
Collection Tubes (2 mL)	30	150
Collection Tubes (1.5 mL)	10	50
Lysis Buffer RLY	6 mL	25 mL
Wash Buffer RW1	2 x 1 mL	15 mL
Wash Buffer RW2	6 mL	12 mL
Membrane Desalting Buffer MEM	10 mL	10 mL
Reaction Buffer for DNase I RDN	7 mL	7 mL
DNase, RNase-free (lyophilized)	1 Vial	1 Vial
Carrier RNA	300 µg	300 µg
Reducing Agent TCEP	14 mg	3 x 14 mg
RNase-free Water	13 mL	13 mL
Bench Protocol Sheet	1	1

Storage & Stability

All kit components should be stored dry and at room temperature. When stored under the recommended conditions and handled correctly, full activity of this kit is retained until the expiry date indicated on the outer box label.