Description
Sweetpotato somatic embryo production is accomplished in two stages. Embryogenic callus is continuously proliferated by subculture on media containing 10 µM 2,4-D and 1 µM 6-BAP. Increa-sing the K+ concentration to 40-60 mM doubled the production of embryogenic callus, while the production of non embry-ogenic callus was reduced by 40%. The development of embryos, trigge-red by the removal of 2,4-D and 6-BAP, was enhan-ced by decrea-sing ammonium (NH4+) from 20 to 10 mM. Sweetpotato somatic embryo production is accomplished in two stages. Embryogenic callus is continuously proliferated by subculture on media containing 10 µM 2,4-D and 1 µM 6-BAP. Increa-sing the K+ concentration to 40-60 mM doubled the production of embryogenic callus, while the production of non embry-ogenic callus was reduced by 40%. The development of embryos, trigge-red by the removal of 2,4-D and 6-BAP, was enhan-ced by decrea-sing ammonium (NH4+) from 20 to 10 mM. Sweetpotato somatic embryo production is accomplished in two stages. Embryogenic callus is continuously proliferated by subculture on media containing 10 µM 2,4-D and 1 µM 6-BAP. Increa-sing the K+ concentration to 40-60 mM doubled the production of embryogenic callus, while the production of non embry-ogenic callus was reduced by 40%. The development of embryos, trigge-red by the removal of 2,4-D and 6-BAP, was enhan-ced by decrea-sing ammonium (NH4+) from 20 to 10 mM.
Composition
| Micro Elements | mg/l | µM |
|---|---|---|
| CoCl2.6H2O | 0.025 | |
| CuSO4.5H2O | 0.025 | |
| FeNaEDTA | 36.70 | |
| H3BO3 | 6.20 | |
| KI | 0.83 | |
| MnSO4.H20 | 16.90 | |
| Na2MoO4.2H2O | 0.25 | |
| ZnSO4.7H2O | 8.60 |
| Macro Elements | mg/l | mM |
|---|---|---|
| CaCl2 | 332.02 | |
| KCl | 2237.00 | |
| KH2PO4 | 170.00 | |
| KNO3 | 2022.00 | |
| MgSO4 | 180.54 | |
| NH4NO3 | 1601.00 |
| Vitamins | mg/l | µM |
|---|---|---|
| myo-Inositol | 90.10 | |
| Nicotinic acid | 1.23 | |
| Pyridoxine HCl | 1.03 | |
| Thiamine HCl | 1.69 |
Total concentration Micro and Macro elements (CP) including -vitamins: 6706.14 mg/l
Cheé R. et al., Optimizing Embryogenic Callus and Embryo Growth of a synthetic seed system for Sweetpotato by varying media nutrient concentrations. J. Am. Soc. Hort. Sci. 117, 663 (1992).
Hazard statements
- H315 Causes skin irritation
- H319 Causes serious eye irritation
- H335 May cause respiratory irritation
Precautionary statements
- P261 Avoid breathing dust/fume/gas/mist/vapours/spray
- P302 IF ON SKIN:
- P305 IF IN EYES:
- P351 Rinse continuously with water for several minutes
- P352 Wash with soap and water
