E.Z.N.A.® Plasmid DNA Mini Kit I, (V-spin)

E.Z.N.A.® Plasmid DNA Mini Kit I, (V-spin)

  • Rapid – Purification of plasmid DNA in less than 30 minutes
  • Safe – No Phenol/chloroform extractions
  • Versatile – Spin and vacuum formats available
  • High-quality – DNA is suitable for a variety of downstream applications

D6943-00S E.Z.N.A.® Plasmid DNA Mini Kit I, (V-spin) (Sample Request)

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Description

The E.Z.N.A.® Plasmid DNA Mini Kit I is designed to isolate up to 25 µg of high-quality plasmid DNA from 1-5 mL bacterial cultures in less than 30 minutes. Plasmid DNA purification follows the alkaline-lysis method and is simplified with HiBind® Mini Column technology into three quick steps: Bind, Wash, and Elute. Purified plasmid DNA is immediately ready for a wide variety of downstream applications such as routine screening, restriction enzyme digestion, transformation, PCR and DNA sequencing.

The E.Z.N.A.® Plasmid DNA Mini Kit is available in two formats: Q-spin (D6942) and V-spin (D6943). D6942 (Q-spin) features columns that are capless, whereas D6943 (V-spin) includes columns that have a cap attached. The columns are otherwise identical in use and application and can be used in both vacuum or centrifugation protocols.

Specifications

For Research Use Only. Not for use in diagnostic procedures.

FEATURES SPECIFICATIONS
Downstream Application Cloning, sequencing, transformation, PCR, restriction digestion, ligation, in vitro transcription etc.
Starting material 1-5 mL LB culture
Plasmid type High-copy, low-copy, cosmid DNA
Processing mode Manual (centrifugation or vacuum)
Throughput 1-24
DNA binding technology Silica Mini Spin Column
Lysate clearance method Centrifugation
Processing time <30 minutes
Yield 15-25 µg for high copy-number; 0.1-5 µg for low copy-number
Special note The alternative for discontinued yeast plasmid kit

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