E-Z 96™ FastFilter Plasmid Kit

E-Z 96™ FastFilter Plasmid Kit

Isolate plasmid DNA from bacterial cultures in 96-well plate format

  • Clear lysate with E-Z 96™ Lysate Clearance Plate
  • No resin, slurries, or alcohol precipitation
  • High-purity plasmid DNA in less than 1 hour
  • Reproducible yields of molecular biology grade plasmid DNA
  • Fast, easy and convenient protocols

D1097-00 E-Z 96™ FastFilter Plasmid Kit (1 x 96 Preps)

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Description

The E-Z 96™ FastFilter Plasmid Kit is optimized for the parallel preparation of up to 96 plasmid minipreps in 1 hour. The Lysate Clearance Plate replaces the time-consuming centrifugation involved in clearing of bacterial alkaline lysates. This kit contains all of the components needed for plasmid DNA purification in a 96-well format. Purified plasmid DNA is suitable for the most sensitive downstream applications, such as routine screening or automated DNA sequencing. The E-Z 96™ Fastfilter Plasmid Kits newly designed 96-well DNA plates are ideal for use with automated liquid handlers.

Specifications

For Research Use Only. Not for use in diagnostic procedures.

FEATURES SPECIFICATION
Downstream Application Cloning; sanger sequencing etc.
Starting material Per well: 1.0-1.5 mL LB culture with OD600 between 2 and 3; or equivalent
Plasmid type High-copy, low-copy, cosmid DNA
Processing mode 96-well, centrifugation or vacuum
Throughput 96 samples per run
DNA binding technology Silica 96-well plate
Lysate clearance method Centrifugation or vacuum with lysate clearnce plate
Processing time <1 hour
Yield 15-25 µg for high copy-number; 0.1-5 µg for low copy-number
Special note Lysate clearance plate for fast processing

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