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HomeShopKits and reagentsMolecular BiologyExtraction KitsCleanupE.Z.N.A.® Cycle Pure Kit (V-spin)
E.Z.N.A.® Cycle Pure Kit (V-spin)

E.Z.N.A.® Cycle Pure Kit (V-spin)

  • Rapid – Purification of PCR products in less than 10 minutes
  • Safe – No Phenol/chloroform extractions
  • Versatile – Spin and vacuum formats available
  • High-quality – DNA is suitable for a variety of downstream applications

D6492-00S E.Z.N.A.® Cycle Pure Kit (V-spin) (Sample Request)

Category:Cleanup Brand: Omega
  • Description
  • Specifications
  • Downloads

Description

The E.Z.N.A.® Cycle Pure Kit is a convenient system for the fast and reliable purification of PCR products. The E.Z.N.A.® Cycle Pure Kit uses HiBind® technology to recover DNA bands from 100 bp to 10 kb free of oligonucleotides, nucleotides, and polymerase with yields exceeding 80%. The binding conditions of the HiBind® DNA Mini Columns are adjusted by the addition of a specially formulated buffer before adding the sample. Following a rapid wash step, DNA is eluted with deionized water or a low salt buffer. Purified DNA can be directly used for most downstream applications include T-A ligations, PCR sequencing, restriction enzyme digestion, or various labeling reactions.

Specifications

For Research Use Only. Not for use in diagnostic procedures.

FEATURES SPECIFICATIONS
Downstream application T-A ligations, sequencing, restriction enzyme digestion, and various other labeling reactions
Elution volume 30-50 µL
Starting material ssDNA, dsDNA, PCR products
Starting amount 5-50 µL
DNA recovered >80% recovery, 100 bp to 10 kb
Processing mode Manual (centrifugation or vacuum)
Throughput 1-24
DNA binding technology Silica mini spin column
Processing time <10 minutes
Special Notes For clean-up of DNA fragments < 200 bp, please follow the protocol modification outlined in the product manual

No downloads provided.

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