SensiFAST™ Probe No-ROX One-Step Kit

Sensitive, reproducible detection of even the most challenging RNA targets, under fast thermal cycling conditions.

Category:Probe One-Step Kits Brand: Bioline

Description

Cat. No.	Size
BIO-76001	100 x 20µl Reactions
BIO-76005	500 x 20µl Reactions

Product Highlights

Sensitive – optimized buffer formulation delivers reliable quantification from even very low copy number RNA targets
Reproducible – consistent results between technical replicates for increased confidence in results
Robust – reliable detection of RNA targets from a broad range of sample types
Fast – delivers reproducible, accurate assay results in as little as 40 minutes
Efficient – excellent performance in multiplex assays
Specific – antibody-mediated hot-start DNA polymerase minimizes non-specific amplification for improved assay sensitivity and reliability

The SensiFAST Probe No-ROX One-Step Kit has been optimized for fast, efficient, unbiased cDNA synthesis and subsequent highly-sensitive, reproducible real-time PCR detection in a single tube. SensiFAST Probe One-Step has been optimized to deliver excellent results in both singleplex and multiplex assays.

An antibody-mediated hot-start DNA polymerase promotes rapid activation and supports highly-specific amplification, which in turn improves assay sensitivity and dynamic range. A combination of the latest advances in buffer chemistry and PCR enhancers confer superior assay performance under fast thermal cycling conditions. The inclusion of separate RiboSafe Inhibitor ensures accuracy by protecting RNA targets from RNase degradation.

The SensiFAST™ Probe No-ROX One-Step Kit has been validated on all commonly-used real-time instruments that do not require the passive reference dye ROX. SensiFAST Probe One-Step has been formulated for use with dual-labelled probes, including TaqMan^®, Scorpions^® and molecular beacon probes.

Applications

Gene expression analysis
Pathogen detection
RNA viral pathogen detection
Genetic profiling
miRNA profiling / quantification
Genetically modified organisms (GMO) characterization

Specifications

Components

Reagent	100 x 20 µL Reactions	500 x 20 µL Reactions
SensiFAST Probe No-ROX One-Step Mix (2x)	1 x 1 mL	5 x 1 mL
RiboSafe RNase Inhibitor	1 x 40 µL	1 x 200 µL
Reverse transcriptase	1 x 20 µL	1 x 100 µL
DEPC-H₂O	1 x 1.8 mL	2 x 1.8 mL

Volume

BIO-76001: 100 x 20 µL Reactions: 1 x 1mL
BIO-76005: 500 x 20 µL Reactions: 5 x 1mL

Storage & Stability

All kit components should be stored at -20°C upon receipt for optimum stability. Repeated freeze/thaw cycles should be avoided.

When stored under the recommended conditions and handled correctly, full activity of the kit is retained until the expiry date indicated on the outer box label.