MyTaq™ Red DNA Polymerase

A new generation of polymerase that delivers improved yield, sensitivity, speed and robustness when amplifying targets from any template.

Category:MyTaq™ DNA Polymerases Brand: Bioline

Description

Cat. No.	Size
BIO-21108	500 Units
BIO-21109	2500 Units
BIO-21110	5000 Units

Product Highlights

Sensitive – incorporates MyTaq DNA polymerase that exhibits increased affinity for DNA thereby improving amplification of even limiting amounts of template
Efficient – novel buffer system maximizes efficiency of PCR amplification, delivering improved yield of any PCR product
Robust – reliable amplification in the presence of inhibitors and with even the most challenging DNA targets
Flexible – ideal for amplifying any target up to 5 kb from DNA extracted from human, animal and plant samples
Convenient – buffer system includes a red dye for improved pipetting ease / accuracy and to enable direct gel loading
Fast – developed to give sensitive, reproducible and robust amplification of a broader range of targets under fast thermal cycling conditions

MyTaq™ Red DNA Polymerase is recommended for all standard PCR applications. The MyTaq DNA Polymerase and MyTaq Red Reaction Buffer in this product, are a unique combination of next-generation polymerase and novel buffer system that deliver very high yield PCR amplification over a wide range of PCR templates. MyTaq has an increased affinity for DNA, enabling reliable amplification from even very low amounts of template. MyTaq DNA Polymerase has been developed to give more robust amplification than other commonly-used polymerases allowing it to perform well with challenging templates and in the presence of PCR inhibitors. Furthermore, the highly efficient nature of MyTaq means it gives excellent results under fast PCR conditions.

The enzyme is supplied with a 5x MyTaq Red Reaction Buffer that increases the visual contrast between the reagent and the reaction vessel for improved convenience and to improve pipetting accuracy. The red dye also enables samples to be loaded directly on to a gel after the PCR without the need to add loading buffer. In addition, the MyTaq Red Reaction Buffer contains dNTPs, MgCl₂ and enhancers at optimal concentrations, which helps eliminate the need for optimization, thereby saving time, effort and the cost of performing unnecessary assay repeats.

Applications

Standard PCR
High-yield PCR
Fast PCR
Colony PCR
TA cloning

Specifications

Components

Reagent	500 Units	2500 Units	5000 Units
MyTaq Red DNA Polymerase	1 x 100 µL	2 x 250 µL	4 x 250 µL
5x MyTaq Reaction Buffer	4 x 1 mL	14 x 1.5 mL	9 x 5 mL

Concentration

5 u/μL

Storage & Stability

MyTaq Red DNA Polymerase can be stored for up to 6 months at -20°C, or up to 2 weeks at +4°C. Repeated freeze/thaw cycles should be avoided. When stored under the recommended conditions and handled correctly, full activity of the kit is retained until the expiry date printed on the outer box label.