MyTaq™ DNA Polymerase

A new generation of polymerase that delivers improved yield, sensitivity, speed and robustness when amplifying targets from any template.

Category:MyTaq™ DNA Polymerases Brand: Bioline

Description

Cat. No.	Size
BIO-21105	500 Units
BIO-21106	2500 Units
BIO-21107	5000 Units

Product Highlights

Sensitive – exhibits increased affinity for DNA, thereby improving amplification of even limiting amounts of template
Efficient – novel buffer system maximizes efficiency of PCR amplification, delivering improved yield of any PCR product
Robust – reliable amplification in the presence of inhibitors and with even the most challenging DNA targets
Flexible – ideal for amplifying any target up to 5 kb, including DNA extracted from human, animal and plant samples
Fast – developed to give sensitive, reproducible and robust amplification of a broader range of targets under fast thermal cycling conditions
Convenient – includes all the components necessary for high performance PCR amplification

MyTaq™ DNA Polymerase is recommended for all standard PCR applications. The MyTaq DNA Polymerase and MyTaq Reaction Buffer in this product, are a unique combination of next-generation polymerase and novel buffer system that deliver very high yield PCR amplification over a wide range of PCR templates. MyTaq has an increased affinity for DNA, enabling reliable amplification from very low amounts of template. MyTaq DNA Polymerase has been developed to give more robust amplification than other commonly-used polymerases allowing it to perform well with challenging templates in the presence of PCR inhibitors.

The composition of the buffer system is critical for efficient PCR. MyTaq reaction buffer contains dNTPs, MgCl₂ and enhancers at optimal concentrations, which helps eliminate the need for optimization, saving time, effort and the cost of performing unnecessary assay repeats.

The combination of MyTaq and optimized buffer system allow for faster PCR reactions compared with other polymerases, therefore reducing overall run time from approximately 1 hour to under 30 minutes. This is achieved without compromising specificity or yield, reducing the reaction time allows for increased throughput and faster time to results.

Applications

Standard PCR
High-yield PCR
Fast PCR
Colony PCR
TA cloning

Specifications

Components

Reagent	500 Units	2500 Units	5000 Units
MyTaq DNA Polymerase	1 x 100 µL	2 x 250 µL	4 x 250 µL
5x MyTaq Reaction Buffer	4 x 1 mL	14 x 1.5 mL	9 x 5 mL

Concentration

5 u/μL

Storage & Stability

All components should be stored at -20°C upon receipt for optimum stability. Repeated freeze/thaw cycles should be avoided.

When stored under the recommended conditions and handled correctly, full activity of the reagents is retained until the expiry date indicated on the outer box label.