Gelnest™ Matrix, For Organ-Like Structures

Introduction

GelNest™ Matrix is derived from mouse tumor tissue and contains extracellular matrix components such as laminin, type IV collagen, heparan sulfate proteoglycans, and more. These components support cell adhesion, differentiation, and proliferation, providing signals for these processes. Additionally, they simulate the characteristics of the basement membrane in the physiological environment, enhancing the success rate and effectiveness of cell culture.

In addition to the matrix components, GelNest™ Matrix is rich in various growth factors. These growth factors promote cell differentiation, proliferation, and migration, mimicking cellular signaling pathways and interactions in the physiological environment. GelNest™ Matrix has a wide range of applications, particularly in tissue engineering, cell culture, and research. It can be used for organoid culture, stem cell differetiation, angiogenesis, migration or invasion assays, and in vivo tumor studies.

 

 

Application

1. 2D Culture Coating 

2. Cell Migration and Tumor Invasion Experiments

Used in combination with NEST Cell Culture Insert to provide a more realistic cellular growth environment.

3.  Organoid Culture

Provides a 3D cell growth environment that is physiologically relevant for studying organ development and function

4. In Vitro/In Vivo Blood Vessel Formation

5. Stem Cell Differentiation

Used for studying stem cell differentiation and tissue regeneration.

6. In Vivo Tumor Formation Experiment

Used for in vivo tumor formation experiments in mice to study tumor growth and treatment.

 

Key Features

1. Protein Concentration

2.  Stable Performance

3. High Safety Performamce 

There are no LDEV (Lactate  Dehydrogenase Elevated Virus), bacteria, or mycoplasma present.

4.  Endotoxin Level

The endotoxin level is <10EU/mL.

5.  Cell Culture

Tests for organ-like structures and stem cell culture have been successfully performed.

6. Experimental Testing

Tests for angiogenesis, tumor invasion, and tumor formation have been successfully conducted.

 

 

Product	Growth Factor	Phenol Red	Recommended Applications	/Pack	/Case	Cat.NO
GelNest™ Matrix	Normal	Yes	General 2D, 3D cell culture	5 mL	1	211212
GelNest™ Matrix, without Phenol Red	Normal	No	Requires colorimetric identification (such as fluorescence) or sensitivity to steroids	5 mL	1	211222
GelNest™ Matrix, low growth factor	Low	Yes	2D, 3D cell culture with higher accuracy	5 mL	1	211232
GelNest™ Matrix, low growth factor, without Phenol Red	Low	No	2D, 3D cell culture with higher accuracy requirements for matrix components and requiresrequirements for matrix components and requires	5 mL	1	211242
GelNest™ Matrix, high concentration	Normal	Yes	colorimetric identification or sensitivity to steroids	5 mL	1	211252
GelNest™ Matrix, high concentration, without Phenol Red	Normal	No	In vivo tumor formation, thrombosis test, angiogenesis experiment, general cell culture, etc.	5 mL	1	211262
GelNest™ Matrix, for stem cells	Low	Yes	hESC stem cell culture	5 mL	1	211272
GelNest™ Matrix, for organ-like structures, without Phenol Red	Low	No	Organoid culture and differentiation	5 mL	1	211282

Storage and Operation Instructions
To store GelNest™ Matrix, it is recommended to keep it in a -20°C freezer until ready for use. Prior to initial use, thaw the gel and divide it  into single-use portions. Store these portions in a -80°C freezer for a maximum of 2 years. Please note that GelNest™ Matrix Gel should not  be stored in a frost-free freezer.
GelNest™ Matrix is in a liquid state at 4°C and transitions into a gel state at 37°C. It starts to solidify into a gel when the temperature exceeds 10°C. For optimal performance, it is advised to pre-chill the pipette tips and conduct the procedures on ice.

In addition to GelNest™ Matrix, Nest delivers the one stop solution for its products including tooling making, injection, processing, sterilization, all under one roof. In addition to this product, NEST has launched over 200 plastic consumable products for cell culture, Molecular Biology, Microbiology, liquid handling and storage, Lab Instruments and Medical Instruments.