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HomeShopKits and reagentsMolecular BiologyExtraction KitsPlasmid DNAE.Z.N.A.® Plasmid DNA Mini Kit I, (Q – spin)
E.Z.N.A.® Plasmid DNA Mini Kit I, (Q – spin)

E.Z.N.A.® Plasmid DNA Mini Kit I, (Q – spin)

  • Rapid – Purification of plasmid DNA in less than 30 minutes
  • Safe – No Phenol/chloroform extractions
  • Versatile – Spin and vacuum formats available
  • High-quality – DNA is suitable for a variety of downstream applications

D6942-00S E.Z.N.A.® Plasmid DNA Mini Kit I, (Q – spin) (Sample Request)

Category:Plasmid DNA Brand: Omega
  • Description
  • Specifications
  • Downloads

Description

The E.Z.N.A.® family of products is an innovative system that radically simplifies the extraction and purification of nucleic acids from a variety of sources. The key to this system is the HiBind® matrix that specifically, but reversibly, binds DNA or RNA under optimized conditions allowing proteins and other contaminants to be removed. The E.Z.N.A.® Plasmid DNA Mini Kits combine the power of HiBind® technology with the time-tested consistency of alkaline-SDS lysis of bacterial cells to deliver high-quality DNA in less than 30 minutes. HiBind® DNA Mini Columns facilitate the binding, washing, and elution steps thus enabling multiple samples to be processed simultaneously.

The E.Z.N.A.® Plasmid DNA Mini Kit is available with two different types of columns. V-spin columns have an attached cap, while Q-spin columns are capless. The columns are otherwise identical in use and application. Both columns can be used with the vacuum or centrifugation protocol.

Specifications

For Research Use Only. Not for use in diagnostic procedures.

FEATURES SPECIFICATIONS
Downstream Application Cloning, sequencing, transformation, PCR, restriction digestion, ligation, in vitro transcription etc.
Starting material 1-5 mL LB culture
Plasmid type High-copy, low-copy, cosmid DNA
Processing mode Manual (centrifugation or vacuum)
Throughput 1-24
DNA binding technology Silica Mini Spin Column
Lysate clearance method Centrifugation
Processing time <30 minutes
Yield 15-25 µg for high copy-number; 0.1-5 µg for low copy-number

No downloads provided.

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