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HomeShopKits and reagentsMolecular BiologyExtraction KitsRNAE.Z.N.A.® Micro RNA Kit
E.Z.N.A.® Micro RNA Kit

E.Z.N.A.® Micro RNA Kit

Isolation of miRNA & large RNA in separate fractions using spin columns

R7034-00S E.Z.N.A.® Micro RNA Kit (Sample Request)

Category:RNA Brand: Omega
  • Description
  • Specifications
  • Downloads

Description

The E.Z.N.A.® Micro RNA Kit combines the reversible binding properties of the HiBind® matrix, a new silica-based material, with the highly efficient lysis ability of RNA-Solv® to extract micro and large (>200 nt) RNA from a wide variety of starting materials. Micro and large RNA are separated to allow for quantification into downstream applications.

The system uses RNA-Solv® to efficiently lyse cells and protect RNA from degradation. Following a chloroform separation step, RNA is applied to a HiBind® RNA column where large RNA binds. The flow-through is retained and applied to a HiBind® Micro RNA column to bind small RNA.

Specifications

For Research Use Only. Not for use in diagnostic procedures.

FEATURES SPECIFICATIONS
Downstream Application RTPCR*, Northern blotting, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation
Elution Volume 15-30 μL for miRNA; 40-70 μL for >200 nt
Starting Material Cultured eukaryotic cells or bacteria, or from animal,
plant, or fungal tissues
Starting Amount Up to 100 mg
RNA Yield Up to 50 µg
Processing Mode Spin column
Throughput 1 – 24
RNA Binding Technology Silica technology
Binding Capacity 100 µg for each

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