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HomeShopKits and reagentsMolecular BiologyExtraction KitsRNAE.Z.N.A.® HP Total RNA Kit
E.Z.N.A.® HP Total RNA Kit

E.Z.N.A.® HP Total RNA Kit

  • Rapid – RNA isolation in 25 minutes
  • Versatile – Spin and vacuum formats available
  • Specialized System – Sample Homogenization and gDNA elimination in one step
  • Safe – No phenol/chloroform extractions
  • High-quality – RNA is suitable for a variety of downstream applications

R6812-00S E.Z.N.A.® HP Total RNA Kit (Sample)

Category:RNA Brand: Omega
  • Description
  • Specifications
  • Downloads

Description

The E.Z.N.A.® HP Total RNA Isolation Kit provides a rapid and easy method for RNA isolation from cultured cells or tissues. The kit includes RNA Homogenizer columns that integrate sample homogenization and genomic DNA elimination into one single step. The RNA purification process is simplified with HiBind® Mini Column technology and can be accomplished in 25 minutes. This convenient spin-column format requires no phenol or chloroform, avoids time-consuming steps such as CsCl gradient ultracentrifugation, precipitation with isopropanol or LiCl and allows for multiple samples to be processed in parallel. RNA purified using the E.Z.N.A.® HP Total RNA method is ready for applications such as RT-PCR, RT-qPCR, Northern blotting, next-generation sequencing, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation.

Specifications

For Research Use Only. Not for use in diagnostic procedures.

FEATURES SPECIFICATIONS
Downstream Application RT-PCR, RT-qPCR, Northern blotting, next-generation sequencing, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation.
Starting Material Cultured cells and tissues
Starting Amount 1 x 107 eukaryotic cells or 25-30 mg tissue
Elution Volume 40-70 μL
RNA Binding Technology Silica Mini spin column
Processing Mode Manual (centrifugation or vacuum)
Throughput 1 – 24
Final Purified Product Total RNA (> 200 nt)
RNA Yield Up to 100 µg
Processing Time 25 min
Special Notes RNA Homogenizer Column that integrates sample homogenization and gDNA elimination into a single step

No downloads provided.

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