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HomeShopKits and reagentsMolecular BiologyExtraction KitsRNAE.Z.N.A.® DNA/RNA Isolation Kit
E.Z.N.A.® DNA/RNA Isolation Kit

E.Z.N.A.® DNA/RNA Isolation Kit

Simultaneously isolate DNA and RNA from same cells or tissues using spin column

R6731-00S E.Z.N.A.® DNA/RNA Isolation Kit (Sample Request)

Category:RNA Brand: Omega
  • Description
  • Specifications
  • Downloads

Description

The E.Z.N.A.® DNA/RNA Isolation Kit is designed for the simultaneous isolation of both genomic DNA and total RNA from the same cells or tissues. The sample is first lysed and homogenized in a special denaturing buffer, spun to pellet RNA and undigested particles, and then the supernatant is applied to a HiBind® DNA spin column to bind DNA. The RNA pellet is dissolved and purified by a HiBind® RNA spin column. Since there is no need to divide the sample into two parts for separate purification procedures, the maximum yield of DNA and RNA can be purified from the entire sample.

Specifications

For Research Use Only. Not for use in diagnostic procedures.

Downstream Application PCR, qPCR, real-time RT-PCR, microarray, Northern blot, poly-A purification
Elution Volume 40-70 µL for RNA; 50-100 µL for DNA
Starting Material Cultured cells and animal tissues
Starting Amount Up to 1X107 cells; up to 30 mg tissue
RNA Yield RNA in one fraction; DNA in another
Processing Mode Manual, centrifugation
Throughput 1 – 24
RNA Binding Technology Silica Mini spin column
Binding Capacity 100 µg for DNA and RNA

No downloads provided.

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