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HomeShopKits and reagentsMolecular BiologyEnd-point PCRStandard PCRNucleotidesdNTP Set
dNTP Set
dNTP Set - Image 1

dNTP Set

The ready-to-use molecular grade dNTP Sets consist of four separate 100 mM solutions of dATP, dCTP, dGTP and dTTP respectively.
Category:Nucleotides Brand: Bioline
  • Description
  • Specifications
  • Downloads

Description

Cat. No. Size
BIO-39025 4 x 25µmol (4 x 250µl)
BIO-39049 4 x 100µmol (4 x 1ml)
BIO-39026 4 x 100µmol (4 x 4 x 250µl)
Product Highlights
  • High quality – >99% purity determined by HPLC, ideal for use in PCR reactions
  • Long shelf-life – no need for aliquoting
  • Convenient formats – available in various concentrations, offering ease of use
  • Enzyme free – DNase, RNase and Nickase free
  • Flexible sizes – custom, bulk and OEM sizes available

Highly quality dNTPs are a vital requirement for successful PCR, as the presence of contaminating impurities will result in a decrease in amplification sensitivity and product yield. Meridian ultra-pure dNTPs undergo highly stringent purification steps to give a greater than 99% purity and are tested for the absence of DNase, RNase, Protease, Nickase activity. They are then quality controlled in a variety of applications, ensuring that they are highly suitable for the most sensitive of PCR techniques.

All Meridian dNTPs are supplied as lithium salts, as lithium salts have greater resistance to repeated freezing and thawing cycles than sodium salts and remain sterile due to the bacteriostatic activity of lithium towards various microorganisms, giving greater reliability and an extended shelf life.

Applications
  • DNA amplification (standard, long-range, multiplex and high-fidelity PCR)
  • DNA labeling
  • RT-PCR
  • qPCR
  • Genotyping
  • cDNA synthesis
  • DNA sequencing

Specifications

Components
Reagent BIO-39025 BIO-39026 BIO-39049
dATP (100 mM)  1 x 250 μL  4 x 250 μL  1 x 1 mL
 dCTP (100 mM)  1 x 250 μL  4 x 250 μL  1 x 1 mL
 dGTP (100 mM)  1 x 250 μL  4 x 250 μL  1 x 1 mL
 dTTP (100 mM)  1 x 250 μL  4 x 250 μL  1 x 1 mL
Concentration
  • 100 mM per tube
Storage & Stability

Ultra-pure dNTPs can be stored for 24 months at -20°C. Avoid multiple freeze/thaw cycles. For long-term storage, aliquoting is recommended.

Products are stable until the expiry date printed on the outer box label

Shipping conditions

On Dry Ice or Blue Ice.

Downloads

dNTP Set - Product Manual

dNTP Set - MSDS

dNTP Set - Fact Sheet

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