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HomeShopKits and reagentsMolecular BiologyEnd-point PCRStandard PCRNucleotidesdNTP Mix
dNTP Mix
dNTP Mix - Image 1

dNTP Mix

Ready-to-use molecular grade dNTP Mixes containing dATP, dCTP, dGTP and dTTP, designed to save hands-on time for researchers and minimize the possibility of contamination.
Category:Nucleotides Brand: Bioline
  • Description
  • Specifications
  • Downloads

Description

Cat. No. Size
BIO-39044 10µmol (1ml)
BIO-39043 20µmol (1 x 500µl)
BIO-39028 50µmol (1 x 500µl)
BIO-39029 200µmol (4 x 500µl)
BIO-39053 100µmol (10 x 1ml)
Product Highlights
  • High quality – >99% purity determined by HPLC, ideal for use in PCR reactions
  • Long shelf-life – no need for aliquoting
  • Convenient formats – available in various concentrations, offering ease of use
  • Enzyme free – DNase, RNase and Nickase free
  • Flexible sizes – custom, bulk and OEM sizes available

Highly quality dNTPs are a vital requirement for successful PCR, as the presence of contaminating impurities will result in a decrease in amplification sensitivity and product yield. Meridian ultra-pure dNTPs undergo highly stringent purification steps to give a greater than 99% purity and are tested or the absence of DNase, RNase, Protease, Nickase activity. They are then quality controlled& in a variety of applications, ensuring that they are highly suitable for the most sensitive of PCR techniques.

All Meridian dNTP Mixes are supplied as an equimolar solution of ultra-pure dATP, dCTP, dGTP and dTTP as lithium salts. Lithium salts have greater resistance to repeated freezing and thawing cycles than sodium salts and remain sterile due to the bacteriostatic activity of lithium towards various microorganisms, giving greater reliability and an extended shelf life.

Applications
  • DNA amplification (standard, long-range, multiplex and high-fidelity PCR)
  • RT-PCR
  • qPCR
  • Genotyping
  • cDNA synthesis
  • DNA sequencing
  • DNA labeling

Specifications

Components
Reagent BIO-39044 BIO-39053 BIO-39043 BIO-39028 BIO-39029
dATP, dCTP, dGTP, dTTP (10 mM)  1 x 1 mL  10 x 1 mL - -  -
dATP, dCTP, dGTP, dTTP (40 mM) - -

 1 x 500 μL

-  -
dATP, dCTP, dGTP, dTTP (100 mM) - - -

 1 x 500 μL

 4 x 500 μL

Concentration
  • BIO-39044 10 mM total
  • BIO-39043 40 mM total
  • BIO-39028 100 mM total
  • BIO-39053 10 mM total
  • BIO-39029 100 mM total
Storage & Stability

All kit components should be stored at -20°C upon receipt for optimum stability. Repeated freeze/thaw cycles should be avoided. For long-term storage, aliquoting is recommended.

When stored under the recommended conditions and handled correctly, full activity of the kit is retained until the expiry date indicated on the outer box label.

Shipping conditions

On Dry Ice or Blue Ice.

Downloads

dNTP Mix - Product Manual

dNTP Mix - Fact Sheet

dNTP Mix - MSDS

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  • dTTP
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