MyTaq™ HS DNA Polymerase

A new generation of hot-start polymerase that delivers improved specificity, yield, speed and robustness when amplifying targets from any template.

Categories:Fast PCR MyTaq™ HS Polymerases & Mixes Brand: Bioline

Description

Cat. No.	Size
BIO-21111	250 Units
BIO-21112	1000 Units
BIO-21113	2500 Units

Product Highlights

Sensitive – exhibits increased affinity for DNA, thereby improving amplification of even limiting amounts of template
Efficient – novel buffer system maximizes efficiency of PCR amplification, delivering improved yield of any PCR product
Specific – an antibody-mediated hot-start enzyme that remains completely inactive during PCR set-up to prevent non-specific amplification
Flexible – ideal for amplifying any target up to 5 kb, including DNA extracted from human, animal and plant samples
Fast – developed to give sensitive, reproducible and robust amplification of a broader range of targets under fast thermal cycling conditions
Convenient – includes all the components necessary for high performance PCR amplification

MyTaq™ HS DNA Polymerase is a new generation of antibody-mediated hot-start enzyme, engineered for highly specific and efficient amplification from even the most challenging templates. MyTaq HS remains inactive at room temperature allowing for convenient reaction set-up, thereby reducing non-specific amplification that can hinder PCR assays from the start. These properties make MyTaq HS DNA Polymerase the ideal choice for PCR assays containing complex and low copy number targets.

The inclusion of dNTPs, MgCl₂and enhancers at optimal concentrations in the buffer helps eliminate the need for optimization, thereby saving time, effort and the cost of performing unnecessary assay repeats. The optimized buffer system and MyTaq HS with its increased affinity for DNA, enables very high yield PCR amplification over a wide range of PCR templates, resulting in reliable amplification from even very low amounts of template.

The advanced formulation of MyTaq HS DNA Polymerase and buffer system has been developed to give more robust amplification than other commonly-used polymerases, meaning it performs reliably even in the presence of PCR inhibitors. Furthermore, it allows fast cycling conditions, considerably reducing the reaction time without compromising PCR specificity or yield.

Applications

Fast PCR
Multiplex PCR
Genotyping
Complex templates (e.g. GC-rich)
Low copy number PCR assays
High-throughput assays with prolonged PCR set-up

Specifications

Components

Reagent	250 Units	1000 Units	2500 Units
MyTaq HS DNA Polymerase	1 x 50 µL	1 x 200 µL	2 x 250 µL
5x MyTaq Reaction Buffer	2 x 1 mL	8 x 1 mL	14 x 1.5 mL

Concentration

5 u/µL

Storage & Stability

All components are shipped on dry/blue ice and should be stored at -20°C upon receipt for optimum stability. Repeated freeze/thaw cycles should be avoided. When stored under the recommended conditions and handled correctly, full activity of the reagents is retained until the expiry date on the outer box label.

Components may also be stored at +4°C if required, although storage at -20°C is recommended. When stored at +4°C, reagents will remain stable for a period of 2 weeks from date of receipt.