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HomeShopKits and reagentsMolecular BiologyEnd-point PCRFast PCRSimpliFi HS Mix
SimpliFi HS Mix
SimpliFi HS Mix - Image 1

SimpliFi HS Mix

A high-fidelity polymerase mix using aptamer-based hot-start technology, highly suited to amplification of DNA from crude samples, for fast, inexpensive target enrichment, NGS library amplification and cloning applications.
Category:Fast PCR Brand: Bioline
  • Description
  • Specifications
  • Downloads

Description

Cat. No. Size
BIO-25060 100 x 50µl Reactions
BIO-25061 500 x 50µl Reactions
Product Highlights
  • Robust – optimized enzyme/buffer mix promotes reliable amplification of a broad range of targets, including complex DNA extracted from human, animal and plant samples
  • Specific – the aptamer-based hot-start remains completely inactive during PCR set-up and after amplification, to prevent non-specific products
  • Optimized – high yields with minimal optimization, regardless of a template’s GC content, reducing time to results and eliminating the cost of unnecessary repeats
  • Enhanced accuracy – higher than 130x Taq fidelity, reducing errors for next generation sequencing (NGS) library amplification

SimpliFi HS Mix is a combination of the latest advances in buffer chemistry and PCR enhancers and stabilizers, together with an aptamer-mediated hot-start polymerase, dNTPs and MgCl2. It has been designed for highly reproducible, accurate assay results in the presence of inhibitors. The advanced buffer chemistry and enhancers has been developed for fast PCR and is designed for superior sensitivity and specificity, making SimpliFi HS Mix perfect for NGS library amplification.

Applications
  • Gene expression
  • Viral and bacterial detection
  • Robust PCR
  • High-specificity PCR
  • High-fidelity PCR
  • GC/AT-rich PCR
  • Blunt end cloning

Specifications

Components
Reagent 100 Reactions 500 Reactions
SimpliFi HS Mix 2 x 1.25 mL 10 x 1.25 mL

 

Volume
  • BIO-25049: 100 x 50 µL Reactions: 2 x 1.25 mL
  • BIO-25050: 500 x 50 µL Reactions: 10 x 1.25 mL
Concentration
  • 2x
Storage & Stability

All components should be stored at -20°C upon receipt for optimum stability. Repeated freeze/thaw cycles should be avoided.

When stored under the recommended conditions and handled correctly, full activity is retained until the expiry date indicated on the outer box label.

Shipping conditions

Shipped on Dry Ice or Blue Ice.

Downloads

SimpliFi HS Mix - Fact Sheet

SimpliFi HS Mix - Product Manual

Simplify HS Mix - MSDS

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