TetroScript Reverse Transcriptase

TetroScript Reverse Transcriptase is a robust thermostable MMLV-RT, designed to reduce RNase H activity and increase thermal stability. The enzyme offers higher cDNA yields and improved efficiency on GC-rich target RNAs and RNA with high secondary structure, such as viral RNA sequences.

Description

Cat. No.	Size
BIO-65111	10,000 Units
BIO-65112	4 x 10,000 Units

Product Highlights

Stable – Reverse transcriptase activity up to 60°C
Robust – Ideal for RNA with high secondary structure such as viral genomes
Sensitive – Detection of low copy number RNA targets
Fast – Ideal for developing fast, highly reproducible RT-qPCR assays

Traditional Moloney Murine Leukemia Virus Reverse Transcriptase (MMLV-RT) is not thermostable and can only maintain its enzymatic activity at relatively low temperatures (up to 50°C). However for cDNA synthesis, a higher reaction temperature is desirable as it reduces RNA secondary structures which can inhibit reverse transcription and it minimizes nonspecific primer binding.

Meridian TetroScript Reverse Transcriptase has higher thermal stability and reduced RNase H activity. The enzyme can be used to synthesize first-strand cDNA at temperatures up to 60°C which improves the cDNA yield from difficult RNA targets that require higher temperature to denature strong secondary structures.

Specifications

Components

Reagent	10,000 units	4 x 10,000 units
TetroScript Reverse Transcriptase	50 µL	4 x 50 µL
5x Reaction Buffer	1 mL	4 x 1 mL

Storage & Stability

TetroScript Reverse Transcriptase can be stored for up to 12 months at -20°C. Repeated freeze/thaw cycles should be avoided.

When stored under the recommended conditions and handled correctly, full activity of the kit is retained until the expiry date printed on the outer box label.