Tetro cDNA Synthesis Kit

Tetro™ cDNA Synthesis Kit contains all the necessary components to generate cDNA from an RNA template. The generated cDNA is suitable for PCR with gene-specific primers or for other downstream applications. The kit contains MMLV Reverse Transcriptase and is suitable for first strand cDNA synthesis, cDNA library construction and the production of templates for PCR amplification.

Category:Reverse Transcription Brand: Bioline

Description

Cat. No.	Size
BIO-65042	30 Reactions
BIO-65043	100 Reactions

Product Highlights

Highly sensitive -for high-quality, full length cDNA from as little as 10 pg of total RNA
Ultra-stable reverse transcriptase -for long genes and rare transcripts
Simple -one-tube, one-step set-up for highly sensitive PCR
Broad dynamic range -10 pg to 5 μg of RNA

Tetro™ cDNA Synthesis Kit contains our highly sensitive MMLV reverse transcriptase, oligo (dT)₁₈ and random hexamer primers and all the necessary components to generate high quality cDNA from RNA templates (fig. 1). The first-strand cDNA generated is ideal for PCR (fig. 2) and can be used in a variety of other applications, such as analyses of cellular RNAs, characterization of RNA splice variants and the generation and cloning of cDNA.

Tetro cDNA Synthesis Kit is optimized for RT reactions over a wide range of total RNA concentrations (10 pg-5 μg), such that long and low-abundance cDNAs can be detected by amplification after cDNA synthesis. The kit contains oligo (dT)₁₈ and random hexamer primers. The kit components are fully optimized to generate maximum yields of full-length cDNA.

Applications

Construction of cDNA libraries
2-step PCR assays
Generation of probes for hybridization
Gene cloning

Specifications

Components

Reagent	30 reactions	100 reactions
5x RT Buffer	120 µL	1.2 mL
Reverse Transcriptase (200 u/µL)	30 µL	100 µL
RNase Inhibitor (10 u/µL)	30 µL	100 µL
dNTP Mix 10 mM Total	30 µL	100 µL
Oligo (dT)₁₈ Primer Mix	30 µL	100 µL
Random Hexamer Primer Mix	30 µL	100 µL
DEPC-treated Water	1.8 mL	1.8 mL

Storage & Stability

All kit components should be stored at -20°C upon receipt. When stored under the recommended conditions and handled correctly, full activity of the kit is retained until the expiry date on the outer box label.