Description
PET Memberane Cell Culture Inserts for 12 well plates With ISO9001&13485 certified, PET Memberane Cell Culture Inserts for 12 well plates are manufactured in 100,000 grade cleaning room with USP 6 grade raw materials. Nest is committed to be your best choice for laboratory and medical consumables.
Nest delivers the one stop solution for its products including tooling making, injection, processing, sterilization, all under one roof. In addition to this product, NEST has launched over 200 plastic consumable products for cell culture, Molecular Biology, Microbiology, liquid handling and storage, Lab Instruments and Medical Instruments.
Application：
co-culture, epithelial cell polarity, migration, invasion, transport and permeability studies.
Permeable supports, also known as cell culture inserts, are an essential tool for the study of both anchorage-dependent and
independent cell lines.
This product produce a cell culture environment that closely resembles an in vivo state. Allow polarized cells to carry out
metabolic activities in a more natural manner because the cells feed both apically and basolaterally.
·Supplied in multiple well plates: 6-well, 12-well, 24well.
·PC membrane: low adsorption rate, reducing the loss of small molecular proteins and other compounds
·Transparent PET inserts permit sufficient optical transparency for visualization of cell outlines by phase contrast microscopy.
·Transparent PET membrane is easy to observe.
·The PC membrane is not easy to be torn or curled, and it is easy to operate when taking out the chamber.
·Compatible with most solvents used for fixing and dyeing.
| packing specification | Inserts/plate | Insert Diameter(mm) | Well Volume(ml) | Insert Volume(ml) | Insert Membrane Growth Area（cm²） |
| 12 | 12 inserts/12well | 12 | 1.5 | 0.5 | 1.12 |
| Pore Size(um) | Membrane Density（well/cm²） |
| 0.4 | 1×108 |
| 1.0 | 2X107 |
| 3.0 | 2×106 |
| 5.0 | 4×105 |
| 8.0 | 1×105 |
| Pore Size(um) | Membrane Density（well/cm²） |
| 0.4 | 1×108 |
| 1.0 | 2X107 |
| 3.0 | 2×106 |
| 5.0 | 4×105 |
| 8.0 | 1×105 |
Cell Culture Insert, PC Membrane
Individually packaged in peelable Tyvek film and Blister, sterile.
| TC Treated Cat.NO. | Non-Treated Cat.NO. | Description | Pore Size
(μm)
|
Membrane Transparence
|
| 723001 | 723011 | 6 Cell Culture Inserts + 6 Well Plate | 3.0 | Translucent
|
| 724001 | 724011 | 12 Cell Culture Inserts + 12 Well Plate | 3.0 | Translucent
|
| 725001 | 725011 | 12 Cell Culture Inserts + 24 Well Plate | 3.0 | Translucent
|
| 723101 | 723111 | 6 Cell Culture Inserts + 6 Well Plate | 0.4 | Opaque
|
| 724101 | 724111 | 12 Cell Culture Inserts + 12 Well Plate | 0.4 | Opaque
|
| 725101 | 725111 | 12 Cell Culture Inserts + 24 Well Plate | 0.4 | Opaque
|
| 724201 | 724211 | 12 Cell Culture Inserts + 12 Well Plate | 5.0 | Translucent
|
| 725201 | 725211 | 12 Cell Culture Inserts + 24 Well Plate | 5.0
|
Translucent
|
| 723301 | 723311 | 6 Cell Culture Inserts + 6 Well Plate | 8.0
|
Translucent
|
| 724301 | 724311 | 12 Cell Culture Inserts + 12 Well Plate | 8.0
|
Translucent
|
| 725301 | 725311 | 12 Cell Culture Inserts + 24 Well Plate | 8.0
|
Translucent
|
| 726001 | / | 100 mm Cell Culture Insert-Dish | 3.0 | Translucent
|
Cell Culture Insert, PET Membrane
Individually packaged in peelable Tyvek film and Blister, sterile.
New addition: 1μm & 8μm PET Membrane
| TC Treated
Cat.NO. |
Non-Treated
Cat.NO. |
Description | Pore Size
(μm)
|
Membrane Transparence
|
Insert Packing
|
Plate Packing
|
| 723421 | 723431 | 6 Cell Culture Inserts + 6 Well Plate | 1.0 | Opaque | 6 | 24 |
| 724421 | 724431 | 12 Cell Culture Inserts + 12 Well Plate | 1.0 | Opaque | 12 | 48 |
| 725421 | 725431 | 12 Cell Culture Inserts + 24 Well Plate | 1.0 | Opaque | 12 | 48 |
| 723321 | 723331 | 6 Cell Culture Inserts + 6 Well Plate | 8.0 |
Transparent
|
6 | 24 |
| 724321 | 724331 | 12 Cell Culture Inserts + 12 Well Plate | 8.0 |
Transparent
|
12 | 48 |
| 724321 | 724331 | 12 Cell Culture Inserts + 24 Well Plate | 8.0 |
Transparent
|
12 | 48 |
• Pre-equilibration: After added culture solution to the pores of the multi-well plate and inserted the insert, please placed it in the incubator for at least one hour or overnight.
• Fix and stain cells directly in the nest, and use a scalpel to cut the membrane for long-term storage. Before using please add culture solution to the pores of the multi-well plate, then insert the insert and add the culture solution which included cells to the insert.
• Before using please add culture solution to the pores of the multi-well plate, then insert the insert and add the culture solution which included cells to the insert.
Before using please add culture solution to the pores of the multi-well plate, then insert the insert and add the culture solution which included cells to the insert.
