Description
A two-fold increase in shoot multiplication of red raspberry on Anderson’s medium was achieved as compared to the Murashige and Skoog formulation. A comparison of the inorganics of both formulations showed a reduction to approximately 1/4 strength on NH4NO3 and KNO3 in Anderson inorganics. The optimal concentrations of growth- regulators for shoot multiplication of red and black raspber-ry were 0.1-2.5 µM IBA and 4.5-9.0 µM BA. In vitro rooting of black and red raspberries were succes-ful using the basal media that included Anderson’s Inorganics, 5µM IBA and 600 mg/l activated charcoal. The latter is essential for high rooting percentagesA two-fold increase in shoot multiplication of red raspberry on Anderson’s medium was achieved as compared to the Murashige and Skoog formulation. A comparison of the inorganics of both formulations showed a reduction to approximately 1/4 strength on NH4NO3 and KNO3 in Anderson inorganics. The optimal concentrations of growth- regulators for shoot multiplication of red and black raspber-ry were 0.1-2.5 µM IBA and 4.5-9.0 µM BA. In vitro rooting of black and red raspberries were succes-ful using the basal media that included Anderson’s Inorganics, 5µM IBA and 600 mg/l activated charcoal. The latter is essential for high rooting percentagesA two-fold increase in shoot multiplication of red raspberry on Anderson’s medium was achieved as compared to the Murashige and Skoog formulation. A comparison of the inorganics of both formulations showed a reduction to approximately 1/4 strength on NH4NO3 and KNO3 in Anderson inorganics. The optimal concentrations of growth- regulators for shoot multiplication of red and black raspber-ry were 0.1-2.5 µM IBA and 4.5-9.0 µM BA. In vitro rooting of black and red raspberries were succes-ful using the basal media that included Anderson’s Inorganics, 5µM IBA and 600 mg/l activated charcoal. The latter is essential for high rooting percentages
Composition
| Micro Elements | mg/l | µM |
|---|---|---|
| CoCl2.6H2O | 0.025 | 0.11 |
| CuSO4.5H2O | 0.025 | 0.10 |
| FeNaEDTA | 73.40 | 200.00 |
| H3BO3 | 6.20 | 100.27 |
| KI | 0.30 | 1.81 |
| MnSO4.H2O | 16.90 | 100.00 |
| Na2MoO4.2H2O | 0.25 | 1.03 |
| ZnSO4.7H2O | 8.60 | 29.91 |
| Macro Elements | mg/l | mM |
|---|---|---|
| CaCl2 | 332.02 | 2.99 |
| KNO3 | 480.00 | 4.75 |
| MgSO4 | 180.54 | 1.50 |
| NaH2PO4 | 330.60 | 2.75 |
| NH4NO3 | 400.00 | 5.00 |
Anderson W.C., Tissue culture propagation of Red and Black Raspberries, Rubus Idaeus and R. Occidentalus Act. Hort., 112, 13 (1980).
Hazard statements
- H315 Causes skin irritation
- H319 Causes serious eye irritation
- H335 May cause respiratory irritation
Precautionary statements
- P261 Avoid breathing dust/fume/gas/mist/vapours/spray
- P302 IF ON SKIN:
- P305 IF IN EYES:
- P351 Rinse continuously with water for several minutes
- P352 Wash with soap and water
