Description
Mag-Bind® Viral DNA/RNA Xpress Kit CE IVD follows a magnetic bead-based approach for the rapid and reliable isolation of viral DNA and RNA from nasopharyngeal (NP) swab specimens that are dry or in viral transport media (VTM), from saliva and other sample sources. The extraction methodology is easily adaptable to various automated systems and can also be scaled up or down depending on the amount of starting sample amount used. The kit utilizes the proven Mag-Bind® technology that enables the purification of high-quality nucleic acids that are free of proteins, nucleases, and other impurities. The purified nucleic acids are ready for direct use in downstream applications such as qPCR, RT-qPCR, and more.
If using the Mag-Bind® Viral DNA/RNA Xpress Kit CE IVD for the first time, please read this manual to become familiar with the procedure. The samples are first lysed in TNA Lysis Buffer under highly denaturing conditions to inactivate the RNases and to preserve the integrity of viral RNA. Carrier RNA is added to the lysis buffer to enhance the binding of viral nucleic acids to the magnetic beads and to maximize the recovery from low viral titer samples. The lysate is then mixed with Mag-Bind® Particles RQ along with isopropanol to bind viral nucleic acids to the magnetic beads. The viral nucleic acid-bound Mag-Bind® Particles RQ are washed twice in 80% ethanol and then eluted in Nuclease-free Water. Please note that the kit is not designed to separate cellular nucleic acids from viral nucleic acids, therefore cellular nucleic acids will be co-purified if present.
