SURFSeq 5000* High-throughput Sequencing Platform

SURFSeq 5000* High-throughput Sequencing Platform

SKU: SQ00025 Category: Brand:

Description

URFSeq 5000* is a bench-top high-throughput sequencing platform with multiple application scenarios, fast sequencing speed, good data quality and low startup cost. Its versatile function can meet the performance requirements of users in whole genome sequencing, targeted sequencing, transcriptome sequencing, metagenomic sequencing, methylation sequencing and other technical fields. SURFSeq 5000* can fully assist end-users no matter in scientific discovery research or clinical diagnosis usage.

Dual flow cell to support 5 different modes/run. Compatible with most common used applications.

 High Accuracy

Unique reversible base termination and sequencing reaction system, combined with a high-sensitivity fluorescence signal detection system to maximize sequencing accuracy.

 Ulter-high sequencing speed

FCM PE150 could finish one run within 24 hours. Flexible FQ upload node, support one or two flow cell in one run.

Specifications

Specification
Flowcell Lane Throughput（Reads/FC） Type Read Length Data output1

(FC×2)

 Q value

(Balanced mode)

 TAT2
FCM 4 500 M 100 cycles SE100 50 Gb×2 Q30≥90%

Q40≥85%

 12 hr
PE50 50 Gb×2 12 hr
200 cycles PE100 100 Gb×2 18 hr
300 cycles PE150 150 Gb×2 22 hr
600 cycles PE300 300 Gb ×2 48 hr
FCH 4 2000 M 50cycles SE50 100 Gb×2 14 hr
100cycles SE100 200 Gb×2 21 hr
PE50 200 Gb×2 21 hr
200cycles PE100 400 Gb×2 26 hr
300cycles PE150 600 Gb×2 30 hr
FCP 4 3600 M 50 cycles SE50 180 Gb×2 16 hr
100 cycles SE100 360 Gb×2 24 hr
200 cycles PE100 720 Gb×2 32 hr
300 cycles PE150 1.1 Tb×2 38 hr
1. The percentage of bases above Q30 and run time is the average of an internal standard library over the entire run. The actual performance is affected by factors such as sample type, library quality, and insert fragment length. Q40 upgrade will be online in 1H 2024.

2. Run time was calculated based on dual flow cell mode, and includes sample loading, sequencing, base calling and data processing.

 

Specification
Dimensions 1090 mm×690 mm×810 mm
Weight 240 kg
Power 2000 VA
Power requirement 100 -240 V ~，50/60 Hz
Operating environment Temperature： 19 ℃-25 ℃

Humidity： 20%-80% relative humidity (no-condensing)

Altitude： below 3000 meter
Computer CPU：Intel Xeon Gold 6342

Memory：256 GB

Hard Drive 1： 2 TB SSD

Hard Drive 2： 17 TB  HDD

System： Windows10 x64

 

Application Read length Data/sample FCM*1 FCM*2 FCH*1 FCH*2 FCP*1 FCP*2
500M 1000M 2000 M 4000M 3600M 7200M
WGS PE150 100Gb/sample 1 2 6 12 10 20
Target panel（FFPE） PE150 5Gb/sample 25 50 100 200 180 360
Target panel

（ctDNA）

 PE50 10Gb/sample 4 8 16 32 30 60
RNAseq PE150 10Gb/sample 12 25 50 100 90 180
WES PE150 10Gb/sample 12 25 50 100 90 180
Single cell PE150 60Gb/sample 2 4 10 20 18 36

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