SensiFAST™ Probe dUTP Mix

RT-qPCR master mix designed to prevent carry-over qPCR contamination making it ideal for use in high-throughput laboratories.

SensiFAST™ Probe dUTP Mix is a fast, highly reproducible real-time PCR master mix that has been designed with a highly optimized buffer chemistry and hot-start DNA polymerase, to deliver excellent results and very low background, allowing greater sensitivity.

Category:Probe Kits Brand: Bioline

Description

Cat. No.	Size
BIO-86201	500 Reactions
BIO-86220	2000 Reactions

Product Highlights

Optimized: dTTP to dUTP ratio optimized for high sensitivity and specificity
Specific: antibody-mediated hot-start DNA polymerase minimizes non-specific amplification for improved assay sensitivity and reliability
Sensitive: reliable quantification of low abundance targets and scarce samples
Reproducible: increased consistency between technical replicates for increased confidence in results
Fast: delivers reproducible, multiplex accurate assay results in as little as 30 minutes

SensiFAST™ Probe dUTP Mix is a 2x, combination of the latest advances in buffer chemistry and PCR enhancers and stabilizers, together with an antibody-mediated hot-start polymerase, dNTPs and MgCl2. However, some of the dTTPs in the dNTP mix has been substituted for dUTP, so that Uracil DNA Glycosylase (UDGase) can be added to remove carry-over qPCR contamination.

The advanced buffer chemistry and enhancers has been developed for fast qPCR and is designed for superior sensitivity and specificity with probe‑detection technology, including TaqMan®, Scorpions® and molecular beacon probes, making SensiFAST Probe dUTP Mix perfect for multiplexing, allowing more samples to be run in a day with the highest confidence, ideal for high‑throughput assays.

Applications

Gene expression analysis
Viral and bacterial detection
GMO testing
SNP genotyping
Mutation detection
Environmental monitoring

Specifications

Components

Reagent	500 x 20 µL Reactions	2000 x 20 µL Reactions
SensiFAST Probe dUTP Mix (2x)	5 x 1 mL	4 x 5 mL

Volume

BIO-86201: 200 x 20 µL Reactions: 5 x 1 mL
BIO-86220: 1000 x 20 µL Reactions: 4 x 5 mL

Storage & Stability

All kit components should be stored at -20°C upon receipt. When stored under the recommended conditions and handled correctly, full activity of the kit is retained until the expiry date on the outer box label.

Avoid exposure of the ROX™ to light.

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