MyTaq™ Extract-PCR Kit

MyTaq Extract-PCR Kit provides quick and easy extraction and amplification of DNA from a variety of tissue types. The MyTaq Extract-PCR Kit maximizes sensitivity while simultaneously minimizing contamination risks to deliver greater experiment success rates.

Categories:Direct PCR Genotyping Kits Brand: Bioline

Description

Cat. No.	Size
BIO-21126	100 Reactions
BIO-21127	500 Reactions

Product Highlights

Fast – single-tube protocol that eliminates wash steps, giving high-yield, PCR-ready DNA in just 15 minutes
Simple – few protocol steps greatly reduce the risk of sample loss and contamination and minimizes manual effort
Sensitive – incorporates MyTaq HS DNA Polymerase that exhibits increased affinity for DNA, thereby improving yield of even the most challenging targets
Specific – MyTaq HS DNA Polymerase is an antibody-mediated hot-start enzyme that remains completely inactive during PCR set-up to prevent non-specific amplification
Flexible – ideal for amplifying any target up to 5 kb from DNA extracted from mammalian tissue samples
Convenient – mastermix facilitates PCR set-up and includes a red dye for improved pipetting ease / accuracy and to enable direct gel loading

Many DNA extraction methods can be laborious and time consuming or involve the use of hazardous chemicals. MyTaq™ Extract-PCR Kit offers a rapid, easy and safer alternative for the extraction and amplification of DNA from a variety of tissue types. MyTaq Extract-PCR Kit is particularly suited to solid tissues such as mouse tail or mouse ear. The DNA extractions are performed in a single-tube, without the need for multiple washing steps, greatly reducing the risk of sample loss and contamination.

The extracted DNA is amplified in a proprietary buffer system using MyTaq HS Red Mix, the latest generation of very high-performance polymerase unique to Meridian. To further reduce non-specific amplification, MyTaq HS uses antibody hot-start technology. The advanced formulation of MyTaq HS Red Mix allows fast cycling conditions to be used, greatly reducing the reaction time without compromising PCR specificity or yield.

The rapid MyTaq Extract-PCR Kit maximizes sensitivity while minimizing contamination risks to deliver improved success rates in applications such as mouse DNA characterization. The single tube lysis protocol and MyTaq HS Red Mix maximize sensitivity, while minimizing contamination risks and significantly reduce reaction times as well as delivering improved success rates in protocols such as mouse DNA characterization

Applications

High-throughput genotyping from mammalian tissue
Detection of transgenes
Knockout analysis

Specifications

Components

Reagent	100 Reactions	500 Reactions
Buffer A	2 x 1 mL	10 x 1 mL
Buffer B	1 x 1 mL	5 x 1 mL
MyTaq HS Red Mix, 2x	1 x 1.25 mL	5 x 1.25 mL

Concentration

QC specifications

Meridian operates under ISO 13485 Management System. MyTaq Extract-PCR Kit and its components are extensively tested for activity, processivity, efficiency, heat activation, sensitivity, absence of nuclease contamination and absence of nucleic acid contamination prior to release.

Storage & Stability

All components should be stored at -20°C upon receipt for optimum stability. Repeated freeze/thaw cycles should be avoided.

When stored under the recommended conditions and handled correctly, full activity of the reagents is retained until the expiry date indicated on the outer box label.