EasyLadder I

Ready-to-use molecular weight marker, designed for fast size determination of nucleic acids from 100 bp to 2000 bp.

Description

Cat. No.	Size
BIO-33045	200 Lanes
BIO-33046	500 Lanes

Product Highlights

Broad size range – 5 evenly distributed bands from 50 bp to 2000 bp
Ready-to-use – reduces handling steps and saves time
Fast separation – ideal for PCR products
Supplied with loading dye – for loading sample DNA

Agarose gels are often run to check and see if a band is present or not, for example in a PCR reaction. With 5 regularly spaced bands, ranging from 100 bp to 2000 bp, EasyLadder™ I is a molecular weight marker especially designed for fast size determination of linear double-stranded DNA fragments on 0.5% to 3% TAE or TBE agarose gels.

This ready-to-use format, with a red loading dye already added to the ladder, reduces handling steps and saves time; simply transfer EasyLadder I from the vial to the gel, along with the PCR product and run at high voltage for just a few minutes (1-3 cm of a gel lane). The concentration of DNA in each of the EasyLadder I bands is provided for optionally determining the approximate mass of DNA in comparably intense sample bands of similar size. The EasyLadder I is also supplied with a 5x loading dye for the loading of sample DNA.

Applications

Determination of PCR amplicon size
Confirmation of plasmid construction
Restriction fragment size determination

Specifications

Components

Reagent	200 Lanes	500 Lanes
EasyLadder	2 x 500 µL	5 x 500 µL
5x Sample Loading Buffer	1 mL	1 mL

Storage & Stability

All components should be stored at -20°C upon receipt for optimum stability. Repeated freeze/thaw cycles should be avoided. When stored under the recommended conditions and handled correctly, full activity of the reagents is retained until the expiry date on the outer box label.

Components may also be stored at +4°C or room temperature if required, although storage at -20°C is always recommended.

When stored at +4°C, EasyLadder I can be stored for up to 12 months. When stored at room temperature, EasyLadder I can be stored for up to 1 month