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HomeShopKits and reagentsMolecular BiologyEnd-point PCRStandard PCRNucleotidesdTTP
dTTP
dTTP - Image 1

dTTP

The ready-to-use molecular grade 100 mM solution of dTTP as lithium salts in purified water.

Category:Nucleotides Brand: Bioline
  • Description
  • Specifications
  • Downloads

Description

Cat. No. Size
BIO-39039 25µmol
Product Highlights
  • High quality – >99% purity determined by HPLC, ideal for use in PCR reactions
  • Long shelf-life – no need for aliquoting
  • Convenient format – ready-to-use concentration
  • Enzyme free – DNase, RNase and Nickase free
  • Flexible sizes – custom, bulk and OEM sizes available

Highly quality dNTPs are a vital requirement for successful PCR, as the presence of contaminating impurities will result in a decrease in amplification sensitivity and product yield. Meridian ultra-pure dTTP undergo highly stringent purification steps to give a greater than 99% purity and are tested for the absence of DNase, RNase, Protease, Nickase activity. It is then quality controlled in a variety of applications, ensuring that it is highly suitable for the most sensitive techniques.

Meridian dTTP is supplied as lithium salts in purified water, as lithium salts have greater resistance to repeated freezing and thawing cycles than sodium salts and remain sterile due to the bacteriostatic activity of lithium towards various microorganisms, giving greater reliability and an extended shelf life.

Applications
  • DNA amplification (standard, long-range, multiplex and high-fidelity PCR)
  • RT-PCR
  • qPCR
  • Genotyping
  • cDNA synthesis
  • DNA sequencing
  • DNA labeling
  • Mutagenesis
  • Expression profiling
  • Epigenetic analysis

Specifications

Components
Reagent 100 mM
dTTP 1 x 250 μL
Concentration
  • 100 mM
Storage & Stability

All components should be stored at -20°C upon receipt for optimum stability. Repeated freeze/thaw cycles should be avoided. For long-term storage, aliquoting is recommended.

When stored under the recommended conditions and handled correctly, full activity of the reagents is retained until the expiry date indicated on the outer box label.

Shipping conditions

Shipped on Dry or Blue ice.

Downloads

dTTP - MSDS

dTTP - Fact Sheet

dTTP - Product Manual

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