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HomeShopKits and reagentsMolecular BiologyReal-Time PCR KitsSensiFast SYBR® KitsSensiFAST™ SYBR® & Fluorescein Kit
SensiFAST™ SYBR® & Fluorescein Kit
SensiFAST™ SYBR® & Fluorescein Kit - Image 1

SensiFAST™ SYBR® & Fluorescein Kit

Outstanding assay reproducibility, sensitivity and robustness from both DNA and RNA templates, under fast thermal cycling conditions.

Category:SensiFast SYBR® Kits Brand: Bioline
  • Description
  • Specifications
  • Downloads

Description

Cat. No. Size
BIO-96005 500 x 20µl Reactions
BIO-96020 2000 x 20µl Reactions
Product Highlights
  • Reproducible – consistent results between technical replicates for increased confidence in results
  • Specific – antibody-mediated hot-start DNA polymerase minimizes non-specific amplification for improved assay sensitivity and reliability
  • Sensitive – reliable quantification of low abundance targets and scarce samples
  • Robust – reliable, accurate detection of DNA and RNA targets from a broad range of sample types
  • Fast – delivers reproducible, accurate assay results in as little as 30 minutes

The SensiFAST™ SYBR & Fluorescein Kit has been developed for fast, highly accurate real-time PCR and has been validated on real-time PCR platforms that require the passive reference dye fluorescein for collection of dynamic well factors.

A combination of the latest advances in buffer chemistry and PCR enhancers ensures that the SensiFAST SYBR & Fluorescein Kit produces reliable assay results under fast thermal cycling conditions.  An antibody-mediated hot-start DNA polymerase system promotes highly-specific amplification, in turn improving assay sensitivity and dynamic range.

The SensiFAST SYBR & Fluorescein Kit has been optimized to deliver optimal performance in tandem with the SensiFAST cDNA Synthesis Kit, which offers fast, unbiased cDNA synthesis, without compromising cDNA yield or coverage.

Applications
  • Gene expression analysis
  • DNA / RNA target detection
  • miRNA profiling / quantification
  • Copy number variation (CNV) analysis

Specifications

Components
Reagent 200 x 20µl Reactions 500 x 20µl Reactions 2000 x 20µl Reactions
SensiFAST SYBR® & Fluorescein mix (2x) 2 x 1ml 5 x 1ml 4 x 5ml
Volume
  • BIO-96002: 250 x 20µl Reactions: 2 x 1ml
  • BIO-96005: 500 x 20µl Reactions: 5 x 1ml
  • BIO-96020: 2000 x 20µl Reactions: 4 x 5ml
Storage & Stability

All kit components should be stored at -20°C upon receipt for optimum stability. Repeated freeze/thaw cycles should be avoided. Avoid exposure of the SYBR® Green I to light.

When stored under the recommended conditions and handled correctly, full activity of the kit is retained until the expiry date indicated on the outer box label.

Shipping conditions

Shipped on dry/blue ice.

Downloads

SensiFAST SYBR & Fluorescein Kit - Manual

SensiFAST SYBR and Fluorescein Kit - MSDS

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