SensiFAST™ SYBR® No-ROX Kit

Outstanding assay reproducibility, sensitivity and robustness from both DNA and RNA templates, under fast thermal cycling conditions.

Category:SensiFast SYBR® Kits Brand: Bioline

Description

Cat. No.	Size
BIO-98005	500 x 20µl Reactions
BIO-98020	2000 x 20µl Reactions
BIO-98050	5000 x 20µl Reactions

Product Highlights

Reproducible – consistent results between technical replicates for increased confidence in results
Specific – antibody-mediated hot-start DNA polymerase minimizes non-specific amplification for improved assay sensitivity and reliability
Sensitive – reliable quantification of low abundance targets and scarce samples
Robust – reliable, accurate detection of DNA and RNA targets from a broad range of sample types
Fast – delivers reproducible, accurate assay results in as little as 30 minutes

The SensiFAST™ SYBR No-ROX Kit has been developed for fast, highly accurate real-time PCR and has been validated on all commonly-used real-time instruments that do not require the passive reference dye ROX.

A combination of the latest advances in buffer chemistry and PCR enhancers ensures that the SensiFAST SYBR No-ROX Kit produces reliable assay results under fast thermal cycling conditions. An antibody-mediated hot-start DNA polymerase system promotes highly-specific amplification, in turn improving assay sensitivity and dynamic range.

The SensiFAST SYBR® No-ROX Kit has been optimized to deliver optimal performance in tandem with the SensiFAST cDNA Synthesis Kit, which offers fast, unbiased cDNA synthesis, without compromising cDNA yield or coverage.

Applications

Gene expression analysis
DNA / RNA target detection
miRNA profiling / quantification
Copy number variation (CNV) analysis

Specifications

Components

Reagent	500 x 20 µL Reactions	2000 x 20 µL Reactions	5000 x 20 µL Reactions
SensiFAST SYBR^® No-ROX mix (2x)	5 x 1 mL	4 x 5 mL	10 x 5 mL

Volume

BIO-98005: 500 x 20 µL Reactions: 5 x 1ml
BIO-98020: 2000 x 20 µL Reactions: 4 x 5ml
BIO-98050: 5000 x 20 µL Reactions: 10 x 5 mL

Storage & Stability

All kit components should be stored at -20°C upon receipt for optimum stability. Repeated freeze/thaw cycles should be avoided. Avoid exposure of the SYBR^® Green I to light.

When stored under the recommended conditions and handled correctly, full activity of the kit is retained until the expiry date indicated on the outer box label.