SensiMix™ SYBR® No-ROX Kit

Outstanding assay reproducibility and sensitivity for both DNA and cDNA templates, under standard conditions.

Category:Sensimix SYBR® kits Brand: Bioline

Description

Cat. No.	Size
QT650-05	500 Reactions

Product Highlights

Sensitive – reproducible detection of low-copy number templates
Reproducible – consistent results between technical replicates for increased confidence in results
Specific – proprietary hot-start modification minimizes non-specific amplification for improved assay reliability in high-throughput assays
Robust – reliable, accurate detection of DNA and cDNA targets from a broad range of sample types

The SensiMix™ SYBR^® No-ROX Kit has been developed for highly accurate real-time PCR and has been validated on real-time PCR platforms that do not require a passive reference dye for normalization of well-to-well differences.

A proprietary, highly-optimized, chemical hot-start PCR enzyme promotes highly-specific amplification, in turn improving assay sensitivity and dynamic range, ensuring that SensiMix SYBR^® No-ROX Kit provide the sensitivity and specificity required for demanding assays under standard thermal cycling conditions.

The SensiMix SYBR^® No-ROX Kit has been optimized to deliver optimal performance in tandem with the SensiFAST cDNA Synthesis Kit, which offers fast, unbiased cDNA synthesis, without compromising cDNA yield or coverage.

Applications

Gene expression analysis
DNA / cDNA target detection
miRNA profiling / quantification
Copy number variation (CNV) analysis

Specifications

Components

Reagent	500 x 50 µL Reactions
SensiMix™ SYBR^® No-ROX (2x)	10 x 1.25 mL (12.5 mL)

Volume

QT650-05: 500 x 50 µL Reactions: 10 x 1.25 mL

Storage & Stability

All kit components should be stored at -20°C upon receipt for optimum stability. Repeated freeze/thaw cycles should be avoided. Avoid exposure of the SYBR^® Green I to light.

When stored under the recommended conditions and handled correctly, full activity of the kit is retained until the expiry date indicated on the outer box label.