{"id":16346,"date":"2025-08-08T11:00:00","date_gmt":"2025-08-08T11:00:00","guid":{"rendered":"https:\/\/filarabiomed.ro\/?post_type=product&#038;p=16346"},"modified":"2025-08-08T11:00:00","modified_gmt":"2025-08-08T11:00:00","slug":"rt-qpcr-extraction-control-red","status":"publish","type":"product","link":"https:\/\/filarabiomed.ro\/en\/product\/rt-qpcr-extraction-control-red\/","title":{"rendered":"RT-qPCR Extraction Control Red"},"content":{"rendered":"<h5 class=\"prodhead\">Product Highlights<\/h5>\n<ul>\n<li>Simple \u2013 streamlined protocol for straightforward validation of RNA extraction and determination of RT-qPCR assay inhibition<\/li>\n<li>Sensitive \u2013 control assay identifies even small effects on RNA extraction and inhibition of amplification<\/li>\n<li>Optimized \u2013 control RNA has a sequence with no known homology to any organism thereby avoiding detection of sample RNA<\/li>\n<li>Specific \u2013 probe-based assay designed specifically for the REC control sequence<\/li>\n<li>Flexible \u2013 ideal for use with a wide range of sample types, including inhibitor-rich samples like blood, urine and sputum sample<\/li>\n<\/ul>\n<p>A common practice in qPCR is to add a known amount of spiked control RNA after RNA extraction, this monitors PCR inhibition but has no value as an extraction control. The ideal situation is to have the test sample and internal control undergo the same processing prior to qPCR. Meridian has developed a RT-qPCR Extraction Control, which more closely mimics the test sample, as compared to spike controls. Genetic material from the test sample and the RT-qPCR Extraction Control is simultaneously extracted by common extraction methods, with the extraction control being as sensitive to inhibition and extraction failure as the test sample.<\/p>\n<p>Artificial RT-qPCR Extraction Control cells are of a known concentration, containing the Internal Control RNA sequence. This sequence contains no known homology to any organism and, importantly, has minimal interference with detection of sample RNA. The RT-qPCR Extraction Control cells are spiked into the lysis buffer with the target sample, prior to RNA extraction. Control Mix, which includes primers and probe, is then added to the reaction mix before amplification. Signal derived from the Internal Control RNA confirms the success of the extraction step. RT-qPCR Extraction Control also monitors co-purification of PCR inhibitors that may cause biased or false amplification patterns.<\/p>\n<h5 class=\"prodhead\">Applications<\/h5>\n<ul>\n<li>Pathogen detection<\/li>\n<li>Cancer risk assessment<\/li>\n<li>Gene expression analysis<\/li>\n<li>Drug therapy efficacy<\/li>\n<li>Biomarker validation<\/li>\n<li>Copy number variation (CNV) analysis<\/li>\n<li>Genotyping<\/li>\n<li>Viral loading<\/li>\n<\/ul>\n","protected":false},"excerpt":{"rendered":"<p>Formerly RNA Extraction Control 670.<\/p>\n<p>RT-qPCR Extraction Control not only enables users of a diagnostic qPCR assay to determine if there are inhibitors in the PCR assay, but also to validate the success of the extraction step, reducing the chance of obtaining a false negative result in the sample RNA.<\/p>\n","protected":false},"featured_media":16347,"template":"","meta":{"inline_featured_image":false},"product_brand":[272],"product_cat":[290],"product_tag":[],"class_list":{"0":"post-16346","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_brand-bioline","7":"product_cat-qpcr-rt-qpcr-extraction-controls","9":"first","10":"instock","11":"shipping-taxable","12":"product-type-simple"},"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v25.5 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>RT-qPCR Extraction Control Red - Filara Biomed<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/filarabiomed.ro\/en\/product\/rt-qpcr-extraction-control-red\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"RT-qPCR Extraction Control Red - Filara Biomed\" \/>\n<meta property=\"og:description\" content=\"Formerly RNA Extraction Control 670.  RT-qPCR Extraction Control not only enables users of a diagnostic qPCR assay to determine if there are inhibitors in the PCR assay, but also to validate the success of the extraction step, reducing the chance of obtaining a false negative result in the sample RNA.\" \/>\n<meta property=\"og:url\" content=\"https:\/\/filarabiomed.ro\/en\/product\/rt-qpcr-extraction-control-red\/\" \/>\n<meta property=\"og:site_name\" content=\"Filara Biomed\" \/>\n<meta property=\"og:image\" content=\"https:\/\/filarabiomed.ro\/wp-content\/uploads\/2025\/06\/rtpcrfig1_550_c31.jpg\" \/>\n\t<meta property=\"og:image:width\" content=\"600\" \/>\n\t<meta property=\"og:image:height\" content=\"600\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/jpeg\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data1\" content=\"2 minutes\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"WebPage\",\"@id\":\"https:\/\/filarabiomed.ro\/en\/product\/rt-qpcr-extraction-control-red\/\",\"url\":\"https:\/\/filarabiomed.ro\/en\/product\/rt-qpcr-extraction-control-red\/\",\"name\":\"RT-qPCR Extraction Control Red - Filara Biomed\",\"isPartOf\":{\"@id\":\"https:\/\/filarabiomed.ro\/en\/#website\"},\"primaryImageOfPage\":{\"@id\":\"https:\/\/filarabiomed.ro\/en\/product\/rt-qpcr-extraction-control-red\/#primaryimage\"},\"image\":{\"@id\":\"https:\/\/filarabiomed.ro\/en\/product\/rt-qpcr-extraction-control-red\/#primaryimage\"},\"thumbnailUrl\":\"https:\/\/filarabiomed.ro\/wp-content\/uploads\/2025\/06\/rtpcrfig1_550_c31.jpg\",\"datePublished\":\"2025-08-08T11:00:00+00:00\",\"breadcrumb\":{\"@id\":\"https:\/\/filarabiomed.ro\/en\/product\/rt-qpcr-extraction-control-red\/#breadcrumb\"},\"inLanguage\":\"en-US\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"https:\/\/filarabiomed.ro\/en\/product\/rt-qpcr-extraction-control-red\/\"]}]},{\"@type\":\"ImageObject\",\"inLanguage\":\"en-US\",\"@id\":\"https:\/\/filarabiomed.ro\/en\/product\/rt-qpcr-extraction-control-red\/#primaryimage\",\"url\":\"https:\/\/filarabiomed.ro\/wp-content\/uploads\/2025\/06\/rtpcrfig1_550_c31.jpg\",\"contentUrl\":\"https:\/\/filarabiomed.ro\/wp-content\/uploads\/2025\/06\/rtpcrfig1_550_c31.jpg\",\"width\":600,\"height\":600},{\"@type\":\"BreadcrumbList\",\"@id\":\"https:\/\/filarabiomed.ro\/en\/product\/rt-qpcr-extraction-control-red\/#breadcrumb\",\"itemListElement\":[{\"@type\":\"ListItem\",\"position\":1,\"name\":\"Home\",\"item\":\"https:\/\/filarabiomed.ro\/en\/\"},{\"@type\":\"ListItem\",\"position\":2,\"name\":\"Shop\",\"item\":\"https:\/\/filarabiomed.ro\/shop\/\"},{\"@type\":\"ListItem\",\"position\":3,\"name\":\"RT-qPCR Extraction Control Red\"}]},{\"@type\":\"WebSite\",\"@id\":\"https:\/\/filarabiomed.ro\/en\/#website\",\"url\":\"https:\/\/filarabiomed.ro\/en\/\",\"name\":\"Filara Biomed\",\"description\":\"\",\"publisher\":{\"@id\":\"https:\/\/filarabiomed.ro\/en\/#organization\"},\"potentialAction\":[{\"@type\":\"SearchAction\",\"target\":{\"@type\":\"EntryPoint\",\"urlTemplate\":\"https:\/\/filarabiomed.ro\/en\/?s={search_term_string}\"},\"query-input\":{\"@type\":\"PropertyValueSpecification\",\"valueRequired\":true,\"valueName\":\"search_term_string\"}}],\"inLanguage\":\"en-US\"},{\"@type\":\"Organization\",\"@id\":\"https:\/\/filarabiomed.ro\/en\/#organization\",\"name\":\"Filara Biomed\",\"url\":\"https:\/\/filarabiomed.ro\/en\/\",\"logo\":{\"@type\":\"ImageObject\",\"inLanguage\":\"en-US\",\"@id\":\"https:\/\/filarabiomed.ro\/en\/#\/schema\/logo\/image\/\",\"url\":\"https:\/\/filarabiomed.ro\/wp-content\/uploads\/2024\/11\/logo-filara.png\",\"contentUrl\":\"https:\/\/filarabiomed.ro\/wp-content\/uploads\/2024\/11\/logo-filara.png\",\"width\":491,\"height\":170,\"caption\":\"Filara Biomed\"},\"image\":{\"@id\":\"https:\/\/filarabiomed.ro\/en\/#\/schema\/logo\/image\/\"}}]}<\/script>\n<!-- \/ Yoast SEO plugin. -->","yoast_head_json":{"title":"RT-qPCR Extraction Control Red - Filara Biomed","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/filarabiomed.ro\/en\/product\/rt-qpcr-extraction-control-red\/","og_locale":"en_US","og_type":"article","og_title":"RT-qPCR Extraction Control Red - Filara Biomed","og_description":"Formerly RNA Extraction Control 670.  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