{"id":16181,"date":"2025-08-08T11:00:00","date_gmt":"2025-08-08T11:00:00","guid":{"rendered":"https:\/\/filarabiomed.ro\/product\/accuzyme-mix\/"},"modified":"2025-08-08T11:00:00","modified_gmt":"2025-08-08T11:00:00","slug":"accuzyme-mix","status":"publish","type":"product","link":"https:\/\/filarabiomed.ro\/en\/product\/accuzyme-mix\/","title":{"rendered":"ACCUZYME\u2122 Mix"},"content":{"rendered":"\n\n\n\n\n
Cat. No.<\/th>\nSize<\/th>\n<\/tr>\n<\/thead>\n
BIO-25028<\/td>\n500 x 50\u00b5l Reactions<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n
\n
\n
Product Highlights<\/span><\/div>\n
\n
    \n
  • Efficient \u2013 highly productive target amplification and removal of 3\u2019 A overhangs<\/li>\n
  • Accurate \u2013 possesses 3\u2019 \u2013 5\u2019 proofreading exonuclease activity that delivers an error rate of 3.0 x 10-6<\/sup> for increased PCR fidelity versus Taq DNA polymerase<\/li>\n
  • Sensitive \u2013 high-yield amplification from limiting amounts of human, animal and plant template DNA<\/li>\n
  • Robust \u2013 developed for reliable amplification of even the most challenging targets, including genomic DNA and GC-rich targets<\/li>\n
  • Flexible \u2013 ideal for amplifying any target up to 5 kb from DNA extracted from mammalian tissue samples<\/li>\n
  • Convenient \u2013 advanced buffering system minimizes the requirements for PCR optimization, thereby reducing time-to-results and eliminating the cost of unnecessary repeats<\/li>\n
  • Simple \u2013 mix reduces pipetting burden for increased consistency in assay set-up and reduced contamination risk<\/li>\n<\/ul>\n<\/div>\n
    \n

    ACCUZYME\u2122 is a proprietary proofreading enzyme that offers increased-fidelity and high PCR yield, even in demanding applications. ACCUZYME has an error-rate of 3.0 x 10-6<\/sup> and results in blunt-ended amplicons up to 5 kb in length, making it ideal for use in cloning and site-directed mutagenesis.<\/p>\n

    ACCUZYME is supplied with a buffering system that provides ideal conditions for most PCR assays. Consequently, the cost and effort typically associated with optimizing assay performance is often eliminated. In circumstances where further optimization is required to improve PCR specificity and\/or yield, ACCUZYME includes an additional vial of MgCl2<\/sub>.<\/p>\n

    ACCUZYME Mix dramatically reduces the time needed to set up reactions, thereby minimizing the risk of contamination. Greater efficiency and reproducibility are achieved by reducing the number of pipetting steps that often lead to variation in reaction set-up.<\/p>\n

    Applications<\/h5>\n
      \n
    • High-fidelity PCR<\/li>\n
    • Routine cloning applications requiring increased PCR yield<\/li>\n
    • Blunt-end cloning<\/li>\n
    • Site-directed mutagenesis<\/li>\n<\/ul>\n<\/div>\n<\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"

      A robust, efficient proofreading enzyme that gives increased fidelity in high-yield PCR, for use in all routine cloning applications.<\/p>\n","protected":false},"featured_media":16226,"template":"","meta":{"inline_featured_image":false},"product_brand":[272],"product_cat":[275],"product_tag":[],"class_list":["post-16181","product","type-product","status-publish","has-post-thumbnail","product_brand-bioline","product_cat-fast-pcr","first","instock","shipping-taxable","product-type-simple"],"yoast_head":"\nACCUZYME\u2122 Mix - Filara Biomed<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/filarabiomed.ro\/en\/product\/accuzyme-mix\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"ACCUZYME\u2122 Mix - Filara Biomed\" \/>\n<meta property=\"og:description\" content=\"A robust, efficient proofreading enzyme that gives increased fidelity in high-yield PCR, for use in all routine cloning applications.\" \/>\n<meta property=\"og:url\" content=\"https:\/\/filarabiomed.ro\/en\/product\/accuzyme-mix\/\" \/>\n<meta property=\"og:site_name\" content=\"Filara Biomed\" \/>\n<meta property=\"og:image\" content=\"https:\/\/filarabiomed.ro\/wp-content\/uploads\/2025\/06\/accuzyme_c_21.jpg\" \/>\n\t<meta property=\"og:image:width\" content=\"458\" \/>\n\t<meta property=\"og:image:height\" content=\"553\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/jpeg\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data1\" content=\"2 minutes\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"WebPage\",\"@id\":\"https:\/\/filarabiomed.ro\/en\/product\/accuzyme-mix\/\",\"url\":\"https:\/\/filarabiomed.ro\/en\/product\/accuzyme-mix\/\",\"name\":\"ACCUZYME\u2122 Mix - 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