{"id":16158,"date":"2025-08-08T11:00:00","date_gmt":"2025-08-08T11:00:00","guid":{"rendered":"https:\/\/filarabiomed.ro\/product\/myfi-mix\/"},"modified":"2025-08-08T11:00:00","modified_gmt":"2025-08-08T11:00:00","slug":"myfi-mix","status":"publish","type":"product","link":"https:\/\/filarabiomed.ro\/en\/product\/myfi-mix\/","title":{"rendered":"MyFi\u2122 Mix"},"content":{"rendered":"\n\n\n\n\n\n
Cat. No.<\/th>\nSize<\/th>\n<\/tr>\n<\/thead>\n
BIO-25049<\/td>\n100 x 50\u00b5l Reactions<\/td>\n<\/tr>\n
BIO-25050<\/td>\n500 x 50\u00b5l Reactions<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n
\n
\n
\n
Product Highlights<\/h5>\n
    \n
  • Robust \u2013 enzyme blend and buffering system promotes reliable amplification of the most challenging and complex targets, even in the presence of inhibitors<\/li>\n
  • Sensitive \u2013 improved target affinity and high processivity ensure successful amplification in low-copy number assays<\/li>\n
  • Efficient \u2013 high-yield amplification of a broad range of targets up to 10 kb including complex DNA extracted from human, animal and plant samples<\/li>\n
  • Specific \u2013 an antibody-mediated hot-start blend that remains completely inactive during PCR set-up to prevent non-specific amplification<\/li>\n
  • Convenient \u2013 an all-in-one-tube mastermix that improves the speed, convenience and accuracy of PCR set-up<\/li>\n
  • Accurate \u2013 proofreading component delivers 3.5x higher fidelity than Taq DNA Polymerase, enabling cloning of PCR products<\/li>\n<\/ul>\n<\/div>\n
    \n

    MyFi\u2122 has been developed to give reliable amplification of targets up to 10 kb from challenging and complex targets. MyFi shows improved tolerance to PCR inhibitors, thereby enabling reliable detection from samples from which DNA is difficult to purify. Furthermore a unique buffer system and enzyme blend promote highly sensitive amplification, ideal for low-copy number targets. The proofreading ability of MyFi allows all PCR products to be cloned. The inclusion of MyTaq HS means MyFi Mix generates PCR products with 3\u2019-A overhangs, which is perfect for TA cloning. MyFi Mix has the added convenience of room temperature reaction assembly, to avoid non-specific amplification and primer-dimer formation.<\/p>\n

    MyFi Mix is supplied as a mastermix that requires the addition of only template, primers and water, thereby reducing the number of pipetting steps during PCR set-up, for improved speed, throughput and assay reproducibility. The inclusion of dNTPs, MgCl2<\/sub> and enhancers at optimal concentrations, helps eliminate the need for optimization, thereby saving on time, cost and making MyFi Mix well suited to automation.<\/p>\n

    Applications<\/h5>\n
      \n
    • Amplification of challenging and complex templates<\/li>\n
    • Longer PCR (up to 10 kb)<\/li>\n
    • Robust PCR<\/li>\n
    • Low-copy PCR<\/li>\n
    • TA cloning<\/li>\n<\/ul>\n<\/div>\n<\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"
      Unique blend of highly-efficient MyTaq HS DNA Polymerase and a proprietary proofreading enzyme that combine to give increased target affinity for use with challenging templates and inhibitor-rich samples.<\/div>\n
      \n
      <\/h5>\n<\/div>\n","protected":false},"featured_media":16231,"template":"","meta":{"inline_featured_image":false},"product_brand":[272],"product_cat":[275],"product_tag":[],"class_list":{"0":"post-16158","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_brand-bioline","7":"product_cat-fast-pcr","9":"first","10":"instock","11":"shipping-taxable","12":"product-type-simple"},"yoast_head":"\nMyFi\u2122 Mix - Filara Biomed<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/filarabiomed.ro\/en\/product\/myfi-mix\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"MyFi\u2122 Mix - Filara Biomed\" \/>\n<meta property=\"og:description\" content=\"Unique blend of highly-efficient MyTaq HS DNA Polymerase and a proprietary proofreading enzyme that combine to give increased target affinity for use with challenging templates and inhibitor-rich samples.\" \/>\n<meta property=\"og:url\" content=\"https:\/\/filarabiomed.ro\/en\/product\/myfi-mix\/\" \/>\n<meta property=\"og:site_name\" content=\"Filara Biomed\" \/>\n<meta property=\"og:image\" content=\"https:\/\/filarabiomed.ro\/wp-content\/uploads\/2025\/06\/myfimixfig1_550_c31.jpg\" \/>\n\t<meta property=\"og:image:width\" content=\"600\" \/>\n\t<meta property=\"og:image:height\" content=\"600\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/jpeg\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data1\" content=\"2 minutes\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"WebPage\",\"@id\":\"https:\/\/filarabiomed.ro\/en\/product\/myfi-mix\/\",\"url\":\"https:\/\/filarabiomed.ro\/en\/product\/myfi-mix\/\",\"name\":\"MyFi\u2122 Mix - 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