{"id":16149,"date":"2025-08-08T11:00:00","date_gmt":"2025-08-08T11:00:00","guid":{"rendered":"https:\/\/filarabiomed.ro\/product\/mytaq-one-step-rt-pcr-kit\/"},"modified":"2025-08-08T11:00:00","modified_gmt":"2025-08-08T11:00:00","slug":"mytaq-one-step-rt-pcr-kit","status":"publish","type":"product","link":"https:\/\/filarabiomed.ro\/en\/product\/mytaq-one-step-rt-pcr-kit\/","title":{"rendered":"MyTaq\u2122 One-Step RT-PCR Kit"},"content":{"rendered":"\n\n\n\n\n\n
Cat. No.<\/th>\nSize<\/th>\n<\/tr>\n<\/thead>\n
BIO-65048<\/td>\n25 Reactions<\/td>\n<\/tr>\n
BIO-65049<\/td>\n100 Reactions<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n
Product Highlights<\/h5>\n
    \n
  • Sensitive \u2013 incorporates a blend of high-affinity RT and novel MyTaq HS DNA Polymerase, enabling amplification of low-copy number targets from \u22653 pg total RNA<\/li>\n
  • Efficient \u2013 novel one-step buffer system maximizes the efficiency of both the reverse transcription and PCR steps, delivering improved yield of any target<\/li>\n
  • Robust \u2013 RT tolerates the higher reaction temperatures required to overcome secondary structure, giving reliable detection of even challenging and GC-rich targets<\/li>\n
  • Specific \u2013 MyTaq HS DNA Polymerase is an antibody-mediated hot-start enzyme that remains completely inactive during PCR set-up to prevent non-specific amplification<\/li>\n
  • Flexible \u2013 utilizes gene-specific primers for full-length reverse transcription and subsequent PCR amplification of any RNA target<\/li>\n
  • Convenient \u2013 an all-in-one-tube mastermix that improves the speed, convenience and accuracy of RT-PCR<\/li>\n<\/ul>\n
    \n

    MyTaq\u2122 One-Step RT-PCR Kit incorporates the latest advances in buffer chemistry, including Meridian ultra-pure dNTPs, together with a proprietary reverse transcriptase and MyTaq HS, a new generation of antibody-mediated hot-start DNA polymerase. This ensures that MyTaq One-Step RT-PCR Kit enables fast, highly-specific and ultra-sensitive amplification of RNA targets for use in a broad range of downstream applications.<\/p>\n

    MyTaq One-Step Kit is ideal for determining the presence or absence of RNA templates and quantifying expression through qualitative or semi-quantitative analysis of RNA transcription levels. The one-step format uses gene specific primers to maximize amplification of these targets, whilst eliminating non-specific amplification, ensuring highly efficient and sensitive transcription from as little as 3 pg total RNA.<\/p>\n

    MyTaq One-Step RT-PCR Kit is perfect for the synthesis of double-stranded cDNA products for subsequent gene expression analysis over a broad temperature range, the use of higher temperatures allows reverse transcription through RNA secondary structure, including difficult and GC-rich sequences. Enhanced amplification of these targets ensures high cDNA yields from all RNA, including total RNA, mRNA, in vitro<\/em> transcribed RNA, snRNA and viral RNA.<\/p>\n

    Applications<\/h5>\n
      \n
    • Gene-expression analysis<\/li>\n
    • Transcription analysis<\/li>\n
    • cDNA cloning<\/li>\n
    • Multiplex RT-PCR<\/li>\n<\/ul>\n<\/div>\n","protected":false},"excerpt":{"rendered":"
      Formulated for highly reproducible first-strand cDNA synthesis and subsequent PCR in a single tube.<\/div>\n
      \n
      <\/h5>\n<\/div>\n","protected":false},"featured_media":16241,"template":"","meta":{"inline_featured_image":false},"product_brand":[272],"product_cat":[277],"product_tag":[],"class_list":["post-16149","product","type-product","status-publish","has-post-thumbnail","product_brand-bioline","product_cat-reverse-transcription","first","instock","shipping-taxable","product-type-simple"],"yoast_head":"\nMyTaq\u2122 One-Step RT-PCR Kit - Filara Biomed<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/filarabiomed.ro\/en\/product\/mytaq-one-step-rt-pcr-kit\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"MyTaq\u2122 One-Step RT-PCR Kit - Filara Biomed\" \/>\n<meta property=\"og:description\" content=\"Formulated for highly reproducible first-strand cDNA synthesis and subsequent PCR in a single tube.\" \/>\n<meta property=\"og:url\" content=\"https:\/\/filarabiomed.ro\/en\/product\/mytaq-one-step-rt-pcr-kit\/\" \/>\n<meta property=\"og:site_name\" content=\"Filara Biomed\" \/>\n<meta property=\"og:image\" content=\"https:\/\/filarabiomed.ro\/wp-content\/uploads\/2025\/06\/mytaq1stepfig1_550_c1.jpg\" \/>\n\t<meta property=\"og:image:width\" content=\"600\" \/>\n\t<meta property=\"og:image:height\" content=\"600\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/jpeg\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data1\" content=\"2 minutes\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"WebPage\",\"@id\":\"https:\/\/filarabiomed.ro\/en\/product\/mytaq-one-step-rt-pcr-kit\/\",\"url\":\"https:\/\/filarabiomed.ro\/en\/product\/mytaq-one-step-rt-pcr-kit\/\",\"name\":\"MyTaq\u2122 One-Step RT-PCR Kit - 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