{"id":16141,"date":"2025-08-08T11:00:00","date_gmt":"2025-08-08T11:00:00","guid":{"rendered":"https:\/\/filarabiomed.ro\/product\/sensifast-cdna-synthesis-kit\/"},"modified":"2025-08-08T11:00:00","modified_gmt":"2025-08-08T11:00:00","slug":"sensifast-cdna-synthesis-kit","status":"publish","type":"product","link":"https:\/\/filarabiomed.ro\/en\/product\/sensifast-cdna-synthesis-kit\/","title":{"rendered":"SensiFAST\u2122 cDNA Synthesis Kit"},"content":{"rendered":"\n\n\n\n\n\n
Cat. No.<\/th>\nSize<\/th>\n<\/tr>\n<\/thead>\n
BIO-65053<\/td>\n50 Reactions<\/td>\n<\/tr>\n
BIO-65054<\/td>\n250 Reactions<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n
\n
\n
Product Highlights<\/span><\/div>\n
\n
    \n
  • Efficient \u2013 high-target affinity, coupled with a novel TransAmp buffer system for improved yield of full-length cDNA<\/li>\n
  • Unbiased \u2013 optimized mix of random hexamers and anchored oligo dT primers for complete 5\u2019 to 3\u2019 RNA sequence representation<\/li>\n
  • Sensitive \u2013 lower Ct values from a broad range of input cDNA concentrations, enabling accurate detection of very low-copy targets<\/li>\n
  • Robust \u2013 reliable reverse transcription under challenging conditions, including complex templates and in the presence of inhibitors<\/li>\n
  • Fast \u2013 high-yield reverse transcription from a very broad of targets in as little as 5 minutes<\/li>\n<\/ul>\n<\/div>\n
    \n

    The SensiFAST\u2122 cDNA Synthesis Kit provides a rapid and sensitive method for first-strand cDNA synthesis, which displays excellent linearity across a wide range of starting material. This gives the same relative representation in cDNA templates, regardless of gene abundance, making it excellent for use in qPCR studies.<\/p>\n

    SensiFAST cDNA Synthesis Kit contains a highly-pure reverse transcriptase and optimized TransAmp\u2122 buffer system, which includes a unique blend of random hexamers and anchored oligo (dT) primers to deliver the highest quality qPCR ready cDNA. This makes the SensiFAST cDNA Synthesis Kit ideal for working with limited sample volumes, such as laser-micro dissected samples and tissue biopsies (down to 1 pg of input RNA), to reverse transcribe precious RNA into stable cDNA ready for accurate real-time quantification. The unique blend of random hexamer primers and anchored oligo dT in the TransAmp Buffer also ensure unbiased 3\u2019 and 5\u2019 coverage and reverse transcription of all regions.<\/p>\n

    SensiFAST cDNA Synthesis Kit can be used with SensiFAST Probe and SensiFAST SYBR\u00ae<\/sup> Kits for fast real-time RT-qPCR without compromising on quality, giving real-time results in less than an hour.<\/p>\n

    Applications<\/h5>\n
      \n
    • Gene expression analysis<\/li>\n
    • Tissue biopsy analysis<\/li>\n
    • miRNA profiling \/ quantification<\/li>\n
    • RNA target detection<\/li>\n
    • Pathogen detection<\/li>\n<\/ul>\n<\/div>\n<\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"

      To complement the SensiFAST\u2122 Probe and SYBR\u00ae<\/sup> kits, Meridian has developed the SensiFAST cDNA Synthesis Kit.<\/p>\n","protected":false},"featured_media":16338,"template":"","meta":{"inline_featured_image":false},"product_brand":[272],"product_cat":[289],"product_tag":[],"class_list":{"0":"post-16141","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_brand-bioline","7":"product_cat-cdna-synthesis-kits","9":"first","10":"instock","11":"shipping-taxable","12":"product-type-simple"},"yoast_head":"\nSensiFAST\u2122 cDNA Synthesis Kit - Filara Biomed<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/filarabiomed.ro\/en\/product\/sensifast-cdna-synthesis-kit\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"SensiFAST\u2122 cDNA Synthesis Kit - Filara Biomed\" \/>\n<meta property=\"og:description\" content=\"To complement the SensiFAST\u2122 Probe and SYBR\u00ae kits, Meridian has developed the SensiFAST cDNA Synthesis Kit.\" \/>\n<meta property=\"og:url\" content=\"https:\/\/filarabiomed.ro\/en\/product\/sensifast-cdna-synthesis-kit\/\" \/>\n<meta property=\"og:site_name\" content=\"Filara Biomed\" \/>\n<meta property=\"og:image\" content=\"https:\/\/filarabiomed.ro\/wp-content\/uploads\/2025\/06\/sfcdna_fig1a1.jpg\" \/>\n\t<meta property=\"og:image:width\" content=\"600\" \/>\n\t<meta property=\"og:image:height\" content=\"600\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/jpeg\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data1\" content=\"2 minutes\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"WebPage\",\"@id\":\"https:\/\/filarabiomed.ro\/en\/product\/sensifast-cdna-synthesis-kit\/\",\"url\":\"https:\/\/filarabiomed.ro\/en\/product\/sensifast-cdna-synthesis-kit\/\",\"name\":\"SensiFAST\u2122 cDNA Synthesis Kit - 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