{"id":16140,"date":"2025-08-08T11:00:00","date_gmt":"2025-08-08T11:00:00","guid":{"rendered":"https:\/\/filarabiomed.ro\/product\/sensifast-hrm-kit\/"},"modified":"2025-08-08T11:00:00","modified_gmt":"2025-08-08T11:00:00","slug":"sensifast-hrm-kit","status":"publish","type":"product","link":"https:\/\/filarabiomed.ro\/en\/product\/sensifast-hrm-kit\/","title":{"rendered":"SensiFAST\u2122 HRM Kit"},"content":{"rendered":"\n\n\n\n\n\n
Cat. No.<\/th>\nSize<\/th>\n<\/tr>\n<\/thead>\n
BIO-32005<\/td>\n500 Reactions<\/td>\n<\/tr>\n
BIO-32020<\/td>\n2000 Reactions<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n
\n
\n
Product Highlights<\/span><\/div>\n
\n
    \n
  • Accurate \u2013 clear discrimination of even the most challenging sequence differences<\/li>\n
  • Reproducible \u2013 unparalleled consistency between replicate difference plots for increased confidence in sample characterization<\/li>\n
  • Sensitive \u2013 efficient detection from even very limiting amounts of sample<\/li>\n
  • Flexible \u2013 enables reliable characterization of a broad range of sequence differences<\/li>\n
  • Fast \u2013 rapid PCR amplification prior to high resolution melting, enabling higher throughput<\/li>\n<\/ul>\n<\/div>\n
    \n

    SensiFAST HRM Kit has been developed for detailed characterization of samples according to their base composition, length and GC content by high resolution melting. The latest advances in buffer chemistry and enhancers, together with an antibody-mediated hot-start DNA polymerase system, ensure the SensiFAST HRM Kit delivers reproducible, accurate HRM analysis.<\/p>\n

    SensiFAST HRM Kit contains EvaGreen\u00ae<\/sup>, a third generation, saturating fluorescent dye which selectively binds to double-stranded DNA. In contrast to dyes such as SYBR\u00ae<\/sup> Green I, EvaGreen can be used at higher concentrations without inhibiting PCR and shows equal binding affinity for GC-rich and AT-rich regions. The combination of our SensiFAST DNA Polymerase, unique buffer system and EvaGreen dye enables amplification and discrimination of even the most challenging sequence differences, such as class IV SNPs without sequence preference. Since it does not require expensive labelled oligonucleotide probes, SensiFAST HRM Kit is also a cost effective alternative to traditional probe based genotyping methods.<\/p>\n

    Applications<\/h5>\n
      \n
    • SNP genotyping<\/li>\n
    • Characterization of haplotype blocks<\/li>\n
    • DNA mapping<\/li>\n
    • Detection of insertions, deletions and translocations<\/li>\n
    • DNA fingerprinting<\/li>\n
    • DNA methylation analysis<\/li>\n
    • Gene scanning<\/li>\n
    • Species identification<\/li>\n
    • Allelic prevalence population studies<\/li>\n
    • Screening for loss of heterozygosity<\/li>\n<\/ul>\n<\/div>\n<\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"

      Precise, highly reproducible and fast genotyping of sequence variants through high resolution melting.<\/p>\n","protected":false},"featured_media":16337,"template":"","meta":{"inline_featured_image":false},"product_brand":[272],"product_cat":[288],"product_tag":[],"class_list":{"0":"post-16140","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_brand-bioline","7":"product_cat-genotyping-kits","9":"first","10":"instock","11":"shipping-taxable","12":"product-type-simple"},"yoast_head":"\nSensiFAST\u2122 HRM Kit - Filara Biomed<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/filarabiomed.ro\/en\/product\/sensifast-hrm-kit\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"SensiFAST\u2122 HRM Kit - Filara Biomed\" \/>\n<meta property=\"og:description\" content=\"Precise, highly reproducible and fast genotyping of sequence variants through high resolution melting.\" \/>\n<meta property=\"og:url\" content=\"https:\/\/filarabiomed.ro\/en\/product\/sensifast-hrm-kit\/\" \/>\n<meta property=\"og:site_name\" content=\"Filara Biomed\" \/>\n<meta property=\"og:image\" content=\"https:\/\/filarabiomed.ro\/wp-content\/uploads\/2025\/06\/sensifasthrm_550_c1.jpg\" \/>\n\t<meta property=\"og:image:width\" content=\"600\" \/>\n\t<meta property=\"og:image:height\" content=\"600\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/jpeg\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data1\" content=\"2 minutes\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"WebPage\",\"@id\":\"https:\/\/filarabiomed.ro\/en\/product\/sensifast-hrm-kit\/\",\"url\":\"https:\/\/filarabiomed.ro\/en\/product\/sensifast-hrm-kit\/\",\"name\":\"SensiFAST\u2122 HRM Kit - 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