{"id":16138,"date":"2025-08-08T11:00:00","date_gmt":"2025-08-08T11:00:00","guid":{"rendered":"https:\/\/filarabiomed.ro\/product\/sensifast-probe-dutp-lo-rox-mix\/"},"modified":"2025-08-08T11:00:00","modified_gmt":"2025-08-08T11:00:00","slug":"sensifast-probe-dutp-lo-rox-mix","status":"publish","type":"product","link":"https:\/\/filarabiomed.ro\/en\/product\/sensifast-probe-dutp-lo-rox-mix\/","title":{"rendered":"SensiFAST\u2122 Probe dUTP Lo-ROX Mix"},"content":{"rendered":"\n\n\n\n\n\n
Cat. No.<\/th>\nSize<\/th>\n<\/tr>\n<\/thead>\n
BIO-84201<\/td>\n500 Reactions<\/td>\n<\/tr>\n
BIO-84220<\/td>\n2000 Reactions<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n
\n
\n
\n
Product Highlights<\/h5>\n
    \n
  • Optimized: dTTP to dUTP ratio optimized for high sensitivity and specificity<\/li>\n
  • Specific: antibody-mediated hot-start DNA polymerase minimizes non-specific amplification for improved assay sensitivity and reliability<\/li>\n
  • Sensitive: reliable quantification of low abundance targets and scarce samples<\/li>\n
  • Reproducible: increased consistency between technical replicates for increased confidence in results<\/li>\n
  • Fast: delivers reproducible, multiplex accurate assay results in as little as 30 minutes<\/li>\n<\/ul>\n<\/div>\n
    \n

    SensiFAST\u2122 Probe dUTP Lo-ROX Mix is a 2x, combination of the latest advances in buffer chemistry and PCR enhancers and stabilizers, together with an antibody-mediated hot-start polymerase, dNTPs and MgCl2. However, some of the dTTPs in the dNTP mix has been substituted for dUTP, so that Uracil DNA Glycosylase (UDGase) can be added to remove carry-over qPCR contamination.<\/p>\n

    The advanced buffer chemistry and enhancers has been developed for fast qPCR and is designed for superior sensitivity and specificity with probe\u2011detection technology, including TaqMan\u00ae, Scorpions\u00ae and molecular beacon probes, and has been validated on all commonly used real-time instruments that require a low concentration of the passive reference dye ROX, making SensiFAST Probe dUTP Lo-ROX Mix perfect for multiplexing, allowing more samples to be run in a day with the highest confidence, ideal for high\u2011throughput assays.<\/p>\n

    Applications<\/h5>\n
      \n
    • Gene expression analysis<\/li>\n
    • Viral and bacterial detection<\/li>\n
    • GMO testing<\/li>\n
    • SNP genotyping<\/li>\n
    • Mutation detection<\/li>\n
    • Environmental monitoring<\/li>\n<\/ul>\n<\/div>\n<\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"

      RT-qPCR master mix designed to prevent carry-over qPCR contamination making it ideal for use in high-throughput laboratories.<\/p>\n

      SensiFAST\u2122 Probe dUTP Lo-ROX Mix is a fast, highly reproducible real-time PCR master mix that has been designed with a highly optimized buffer chemistry and hot-start DNA polymerase, to deliver excellent results and very low background, allowing greater sensitivity.\n<\/p>\n

      \n
      <\/h5>\n<\/div>\n","protected":false},"featured_media":16300,"template":"","meta":{"inline_featured_image":false},"product_brand":[272],"product_cat":[283],"product_tag":[],"class_list":{"0":"post-16138","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_brand-bioline","7":"product_cat-probe-kits","9":"first","10":"instock","11":"shipping-taxable","12":"product-type-simple"},"yoast_head":"\nSensiFAST\u2122 Probe dUTP Lo-ROX Mix - Filara Biomed<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/filarabiomed.ro\/en\/product\/sensifast-probe-dutp-lo-rox-mix\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"SensiFAST\u2122 Probe dUTP Lo-ROX Mix - Filara Biomed\" \/>\n<meta property=\"og:description\" content=\"RT-qPCR master mix designed to prevent carry-over qPCR contamination making it ideal for use in high-throughput laboratories. 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